Analysis of Staphylococcus aureus cell viability, sublethal injury and death induced by synergistic combination of ultrasound and mild heat

被引：81

作者：

Li, Jiao ^{[1
]}

Suo, Yuanjie ^{[1
]}

Liao, Xinyu ^{[1
]}

Ahn, Juhee ^{[2
]}

Liu, Donghong ^{[1
]}

Chen, Shiguo ^{[1
]}

Ye, Xingqian ^{[1
]}

Ding, Tian ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Food Sci & Nutr, Zhejiang Key Lab Agrofood Proc, Hangzhou, Zhejiang, Peoples R China

[2] Kangwon Natl Univ, Dept Med Biomat Engn, Chunchon, South Korea

来源：

ULTRASONICS SONOCHEMISTRY | 2017年 / 39卷

基金：

中国国家自然科学基金;

关键词：

Staphylococcus aureus; Cell viability; Sublethal injury; Ultrasound; Mild heat; ACIDIC ELECTROLYZED WATER; ESCHERICHIA-COLI; POWER ULTRASOUND; DIRECT FUNCTIONALIZATION; GRAPHENE OXIDE; INDUCED DAMAGE; LISTERIA-MONOCYTOGENES; ROBUST APPROACH; FLOW-CYTOMETRY; INACTIVATION;

D O I：

10.1016/j.ultsonch.2017.04.019

中图分类号：

O42 [声学];

学科分类号：

070206 ; 082403 ;

摘要：

This study was designed to investigate the combined effects of ultrasound and mild heat on the viability of S. aureus in association with the cell membrane integrity and intracellular enzyme activity. Cells were treated by ultrasound under 55 degrees C for 3, 5, 7, 10, and 15 min. The dynamic changes of S. aureus cell viability, sublethal injury and death were evaluated using flow cytometric assay. Microscopies were applied to identify the morphological appearance, ultrastructure and topography of S. aureus. The results showed the membrane damage was synchronous with esterase inhibition during the exposure to sonication, leading to the immediate lethal effect. On the other hand, bacteria under the mild heat at 55 degrees C were inactivated via a sublethal injury process. The different lethal modes were observed between sonication and mild heat treatments, which could synergistically inactivate S. aureus. The antibacterial value of thermo-sonication was greater than the sum of the individual treatments. The thermo-sonication combination synergistically reduced the number of sublethal cells and also resulted in severe cell damage.

引用

页码：101 / 110

页数：10

共 47 条

[1] Inactivation of Salmonella enterica serovar enteritidis by ultrasonic waves under pressure at different water activities [J].