Biological Magnetometry: Torque on Superparamagnetic Beads in Magnetic Fields

被引：62

作者：

van Oene, Maarten M. ^{[1
]}

Dickinson, Laura E. ^{[1
]}

Pedaci, Francesco ^{[1
,2
]}

Kober, Mariana ^{[1
]}

Dulin, David ^{[1
]}

Lipfert, Jan ^{[1
,3
,4
]}

Dekker, Nynke H. ^{[1
]}

机构：

[1] Delft Univ Technol, Kavli Inst Nanosci, Dept Bionanosci, NL-2628 CJ Delft, Netherlands

[2] CNRS, UMR 5048, Ctr Biochim Struct, Dept Single Mol Biophys, Montpellier, France

[3] Univ Munich, Nanosyst Initiat Munich, Dept Phys, D-80799 Munich, Germany

[4] Univ Munich, Ctr NanoSci, D-80799 Munich, Germany

来源：

PHYSICAL REVIEW LETTERS | 2015年 / 114卷 / 21期

关键词：

TWEEZERS; PARTICLES; DNA; SPECTROSCOPY; ANISOTROPY; NANOPARTICLES; PROTEINS; SYSTEMS; MOTION; TWIST;

D O I：

10.1103/PhysRevLett.114.218301

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

Superparamagnetic beads are widely used in biochemistry and single-molecule biophysics, but the nature of the anisotropy that enables the application of torques remains controversial. To quantitatively investigate the torques experienced by superparamagnetic particles, we use a biological motor to rotate beads in a magnetic field and demonstrate that the underlying potential is pi periodic. In addition, we tether a bead to a single DNA molecule and show that the angular trap stiffness increases nonlinearly with magnetic field strength. Our results indicate that the superparamagnetic beads' anisotropy derives from a nonuniform intrabead distribution of superparamagnetic nanoparticles.

引用

页数：6

共 42 条

[1] Thermodynamic properties of magneto-anisotropic nanoparticles [J].

Bandyopadhyay, Malay .

JOURNAL OF PHYSICS-CONDENSED MATTER, 2009, 21 (23)

[2] EDDY-CURRENT METHOD FOR MEASURING THE RESISTIVITY OF METALS [J].

BEAN, CP ;

DEBLOIS, RW ;

NESBITT, LB .

JOURNAL OF APPLIED PHYSICS, 1959, 30 (12) :1976-1980

[3] Flagellar Hook Flexibility Is Essential for Bundle Formation in Swimming Escherichia coli Cells [J].

Brown, Mostyn T. ;

Steel, Bradley C. ;

Silvestrin, Claudio ;

Wilkinson, David A. ;

Delalez, Nicolas J. ;

Lumb, Craig N. ;

Obara, Boguslaw ;

Armitage, Judith P. ;

Berry, Richard M. .

JOURNAL OF BACTERIOLOGY, 2012, 194 (13) :3495-3501

[4] Structural transitions and elasticity from torque measurements on DNA [J].

Bryant, Z ;

Stone, MD ;

Gore, J ;

Smith, SB ;

Cozzarelli, NR ;

Bustamante, C .

NATURE, 2003, 424 (6946) :338-341

[5] Magnetic behaviour of iron oxide nanoparticles dispersed in a silica matrix [J].

Caizer, C ;

Savii, C ;

Popovici, M .

MATERIALS SCIENCE AND ENGINEERING B-SOLID STATE MATERIALS FOR ADVANCED TECHNOLOGY, 2003, 97 (02) :129-134

[6] Magnetic Tweezers Measurement of Single Molecule Torque [J].

Celedon, Alfredo ;

Nodelman, Ilana M. ;

Wildt, Bridget ;

Dewan, Rohit ;

Searson, Peter ;

Wirtz, Denis ;

Bowman, Gregory D. ;

Sun, Sean X. .

NANO LETTERS, 2009, 9 (04) :1720-1725

[7] DETERMINATION OF THE MAGNETIC-ANISOTROPY OF FERROFLUIDS FROM TORQUE MAGNETOMETRY DATA [J].

CHANTRELL, RW ;

TANNER, BK ;

HOON, SR .

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 1983, 38 (01) :83-92

[8] Improved High-Force Magnetic Tweezers for Stretching and Refolding of Proteins and Short DNA [J].

Chen, Hu ;

Fu, Hongxia ;

Zhu, Xiaoying ;

Cong, Peiwen ;

Nakamura, Fumihiko ;

Yan, Jie .

BIOPHYSICAL JOURNAL, 2011, 100 (02) :517-523

[9] Thermal fluctuations of magnetic nanoparticles: Fifty years after Brown [J].

Coffey, William T. ;

Kalmykov, Yuri P. .

JOURNAL OF APPLIED PHYSICS, 2012, 112 (12)

[10] Magnetic Forces and DNA Mechanics in Multiplexed Magnetic Tweezers [J].

De Vlaminck, Iwijn ;

Henighan, Thomas ;

van Loenhout, Marijn T. J. ;

Burnham, Daniel R. ;

Dekker, Cees .

PLOS ONE, 2012, 7 (08)

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