Toxoplasma gondii: Identification and immune response against a group of proteins involved in cellular invasion

被引：4
|
作者：
Azzouz, Samira [1 ]
Maache, Mimoun [2 ]
Osuna, Antonio [2 ]
Lawton, Philippe [1 ]
Petavy, Anne Francoise [1 ]
机构：
[1] Univ Lyon 1, Fac Pharm, ISPB, Dept Parasitol & Med Mycol, F-69373 Lyon 08, France
[2] Fac Sci, Dept Parasitol, Inst Biotechnol, Granada 18071, Spain
来源：
EXPERIMENTAL PARASITOLOGY | 2012年 / 130卷 / 01期
关键词：
Toxoplasma gondii; Permeability; Cellular invasion; Vaccine; PROTECTIVE IMMUNITY; MEDIATED-IMMUNITY; INFECTION; CELLS; CD4+; DIFFERENTIATION; PERMEABILITY;
D O I：
10.1016/j.exppara.2011.09.012
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Toxoplasma gondii is an ubiquitous intracellular parasite, causative agent of toxoplasmosis, and a worldwide zoonosis for which an effective vaccine is needed. A group of proteins secreted by tachyzoites during host-cell invasion was isolated from the interaction medium. It induced the permeability of the cells as assessed by alpha-sarcin and consequently facilitated the entry of the parasite into the cells. SDS-PAGE of the purified proteins showed a pattern of four proteins of 67, 42, 32 and 27 kDa. MRC-5 cells incubated with the total protein and the different electroeluted bands endured a high cellular death in presence of alpha-sarcin. BALb/C mice immunized with the group of proteins had a mixed Th1/Th2 response and were protected upon challenge with the parasites. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：63 / 68
页数：6
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