A novel virus-encoded nucleocytoplasmic shuttling protein: The UL3 protein of herpes simplex virus type 1

被引：6
|
作者：
Zheng, Chunfu [1 ]
Lin, Fusen [1 ]
Wang, Shuai [1 ]
Xing, Junji [1 ]
机构：
[1] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Mol Virol & Viral immunol Res Grp, Wuhan 430071, Peoples R China
来源：
JOURNAL OF VIROLOGICAL METHODS | 2011年 / 177卷 / 02期
基金：
中国国家自然科学基金;
关键词：
HSV-1; UL3; Nuclear localization signal; Nuclear export signal; Ran-GTP; Chromosomal region maintenance 1; NUCLEAR EXPORT SIGNAL; OPEN READING FRAME; LOCALIZATION; IDENTIFICATION; TRANSPORT; THERAPY; IMPORT; GENOME; GENES; U(L)3;
D O I：
10.1016/j.jviromet.2011.08.004
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Herpes simplex virus type 1 (HSV-1) UL3 protein is a nuclear protein. In this study, the molecular mechanism of the subcellular localization of UL3 was characterized by fluorescence microscopy in living cells. A nuclear localization signal (NLS) and a nuclear export signal (NES) were also identified. UL3 was demonstrated to target to the cytoplasm through the NES via chromosomal region maintenance 1 (CRM-1) dependent pathway, and to the nucleus through RanGTP-dependent mechanism. Heterokaryon assays confirmed that UL3 was capable of shuttling between the nucleus and the cytoplasm. These results demonstrate that the UL3 protein is a novel HSV-1 encoded nucleocytoplasmic shuttling protein. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码：206 / 210
页数：5
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