Chemical constituents of Triticum aestivum and their effects on adipogenic differentiation of 3T3-L1 preadipocytes

被引：24

作者：

Bui Thi Thuy Luyen ^{[1
]}

Nguyen Phuong Thao ^{[1
,2
]}

Bui Huu Tai ^{[1
,2
]}

Lim, Ji Young ^{[3
,4
]}

Ki, Hyeon Hui ^{[5
,6
]}

Kim, Dae Ki ^{[5
,6
]}

Lee, Young Mi ^{[3
,4
]}

Kim, Young Ho ^{[1
]}

机构：

[1] Chungnam Natl Univ, Coll Pharm, Taejon 305764, South Korea

[2] Vietnam Acad Sci & Technol, Inst Marine Biochem IMBC, Hanoi, Vietnam

[3] Wonkwang Univ, Dept Oriental Pharm, Coll Pharm, Iksan 570749, South Korea

[4] Wonkwang Oriental Med Res Inst, Iksan 570749, South Korea

[5] Chonbuk Natl Univ, Dept Immunolgy, Jeonju 561756, Jeonbuk, South Korea

[6] Chonbuk Natl Univ, Inst Med Sci, Sch Med, Jeonju 561756, Jeonbuk, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2015年 / 38卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

Triticum aestivum; Wheat sprout; Glycolipid; 3T3-L1; preadipocytes; Anti-obesity; ANTIOXIDANT ACTIVITY; TNF-ALPHA; ISOORIENTIN; SIBUTRAMINE; SPROUTS;

D O I：

10.1007/s12272-014-0478-2

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

In this report, we investigated the anti-obesity effect of wheat sprouts and their component compounds. Twenty compounds (1-20) were isolated from Triticum aestivum. Among them, glycolipids 1-5 were determined for the first time from T. aestivum and its sprouts. The HPLC analysis demonstrated that compounds 1-3, 5, 8, 12, and 14 were major peak in the HPLC chromatogram of the active fraction. The effects of the compounds on lipid accumulation were assessed at concentrations ranging from 1.0 to 100 mu M. At concentration of 10.0 mu M, compounds 1-7, 10-15, and 17-19 significantly decreased lipid accumulation in 3T3-L1 preadipocytes. Glycolipids 1, 2, and phenolic 17 significantly reduced lipid accumulation in the differentiated adipocytes in a concentration-dependent manner. Quantitative analysis based on measurement of the optical density of Oil Red O indicated that, at 100 mu M, compounds 1, 2, and 17 reduced lipid accumulation by 41, 37, and 48 %, respectively, compared with the positive control.

引用

页码：1011 / 1018

页数：8

共 29 条

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