Optimization of Inexpensive Agricultural By-Products as Raw Materials for Bacitracin Production in Bacillus licheniformis DW2

被引：24

作者：

Wang, Qin ^{[1
]}

Zheng, Hui ^{[2
]}

Wan, Xia ^{[3
]}

Huang, Hongfeng ^{[3
]}

Li, Junhui ^{[1
]}

Nomura, Christopher T. ^{[1
,4
]}

Wang, Changjun ^{[5
]}

Chen, Shouwen ^{[1
,2
]}

机构：

[1] Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan 430062, Hubei, Peoples R China

[2] Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China

[3] Chinese Acad Agr Sci, Oilcrops Res Inst, Wuhan 430062, Hubei, Peoples R China

[4] SUNY Coll Environm Sci & Forestry SUNY ESF, Dept Chem, Syracuse, NY 13210 USA

[5] Tobacco Res Inst Hubei Prov, Wuhan 430062, Hubei, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2017年 / 183卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Bacitracin; Agricultural by-products; Bacillus licheniformis; Fermentation; Amino acids; OIL CAKE; BIOSYNTHESIS; ANTIBIOTICS; BIOHYDROGEN; MECHANISM; COMPLEX; ACID;

D O I：

10.1007/s12010-017-2489-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacitracin is a broad-spectrum antibiotic used extensively as a feed additive. In this study, inexpensive agricultural by-products were used as nitrogen sources for bacitracin production. Based on both the orthogonal tests, a combination of 7% soybean meal (SBM) +2% low protein rapeseed cake (LPRC) was optimal for bacitracin production. Compared to the original formula, the titer of bacitracin increased by 20.5% reaching 910.4 U/ml in flasks. The titer of bacitracin and the ratio of bacitracin A increased by 12.4 and 6.8% in a 50-l fermentor. Furthermore, this study also explored the effects of exogenously adding different amino acids on the yield of bacitracin. The addition of Cys and Glu enhanced bacitracin production by 5.7 and 5.0%, respectively. This study provided the inexpensive nutrient inputs into efficient bacitracin production and also the insight to further research enabling better utilization of oil cakes for economic viability of the bioprocess industry.

引用

页码：1146 / 1157

页数：12

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