Metabolic pathway visualization in living yeast by DNP-NMR

被引：82
作者：
Meier, Sebastian [1 ]
Karlsson, Magnus [2 ]
Jensen, Pernille R. [2 ]
Lerche, Mathilde H. [2 ]
Duus, Jens O. [1 ]
机构：
[1] Carlsberg Lab, DK-1799 Copenhagen, Denmark
[2] Albeda Res, DK-1799 Copenhagen, Denmark
来源：
MOLECULAR BIOSYSTEMS | 2011年 / 7卷 / 10期
关键词：
MAGNETIC-RESONANCE; IN-VIVO; FLUX ANALYSIS; KINETICS; GLYCOLYSIS; PHOSPHATES; TUMORS;
D O I：
10.1039/c1mb05202k
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Central carbon metabolism of living Saccharomyces cerevisiae is visualized by DNP-NMR. Experiments are conducted as real time assays that detect metabolic bottlenecks, pathway use, reversibility of reactions and reaction mechanisms in vivo with subsecond time resolution.
引用
收藏
页码：2834 / 2836
页数：3
相关论文
共 22 条
[1]   Increase in signal-to-noise ratio of >10,000 times in liquid-state NMR [J].
Ardenkjaer-Larsen, JH ;
Fridlund, B ;
Gram, A ;
Hansson, G ;
Hansson, L ;
Lerche, MH ;
Servin, R ;
Thaning, M ;
Golman, K .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (18) :10158-10163
[2]   ANOMALOUS MUTAROTATION OF GLUCOSE 6-PHOSPHATE - AN EXAMPLE OF INTRAMOLECULAR CATALYSIS [J].
BAILEY, JM ;
FISHMAN, PH ;
PENTCHEV, PG .
BIOCHEMISTRY, 1970, 9 (05) :1189-&
[3]  
Brachmann CB, 1998, YEAST, V14, P115
[4]   Magnetic resonance imaging of pH in vivo using hyperpolarized 13C-labelled bicarbonate [J].
Gallagher, Ferdia A. ;
Kettunen, Mikko I. ;
Day, Sam E. ;
Hu, De-En ;
Ardenkjær-Larsen, Jan Henrik ;
in't Zandt, Rene ;
Jensen, Pernille R. ;
Karlsson, Magnus ;
Golman, Klaes ;
Lerche, Mathilde H. ;
Brindle, Kevin M. .
NATURE, 2008, 453 (7197) :940-U73
[5]   Production of hyperpolarized [1,4-13C2]malate from [1,4-13C2]fumarate is a marker of cell necrosis and treatment response in tumors [J].
Gallagher, Ferdia A. ;
Kettunen, Mikko I. ;
Hu, De-En ;
Jensen, Pernille R. ;
in't Zandt, Rene ;
Karlsson, Magnus ;
Gisselsson, Anna ;
Nelson, Sarah K. ;
Witney, Timothy H. ;
Bohndiek, Sarah E. ;
Hansson, Georg ;
Peitersen, Torben ;
Lerche, Mathilde H. ;
Brindle, Kevin M. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (47) :19801-19806
[6]   Metabolic imaging by hyperpolarized 13C magnetic resonance imaging for in vivo tumor diagnosis [J].
Golman, Klaes ;
in't Zandt, Rene ;
Lerche, Mathilde ;
Pehrson, Rikard ;
Ardenkjaer-Larsen, Jan Henrik .
CANCER RESEARCH, 2006, 66 (22) :10855-10860
[7]   NMR for microbiology: In vivo and in situ applications [J].
Grivet, Jean-Philippe ;
Delort, Anne-Marie .
PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY, 2009, 54 (01) :1-53
[8]   Kinetics of hyperpolarized 13C1-pyruvate transport and metabolism in living human breast cancer cells [J].
Harris, Talia ;
Eliyahu, Galit ;
Frydman, Lucio ;
Degani, Hadassa .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (43) :18131-18136
[9]   Tissue-specific Short Chain Fatty Acid Metabolism and Slow Metabolic Recovery after Ischemia from Hyperpolarized NMR in Vivo [J].
Jensen, Pernille R. ;
Peitersen, Torben ;
Karlsson, Magnus ;
in't Zandt, Rene ;
Gisselsson, Anna ;
Hansson, Georg ;
Meier, Sebastian ;
Lerche, Mathilde H. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (52) :36077-36082
[10]   Hyperpolarized Amino Acids for In Vivo Assays of Transaminase Activity [J].
Jensen, Pernille R. ;
Karlsson, Magnus ;
Meier, Sebastian ;
Duus, Jens O. ;
Lerche, Mathilde H. .
CHEMISTRY-A EUROPEAN JOURNAL, 2009, 15 (39) :10010-10012
123