Changes of amino acid concentrations in the rat vestibular nuclei after midline lesions

被引:1
作者
Sun, Yizhe [2 ]
Godfrey, Donald A. [1 ]
Godfrey, Matthew A. [1 ]
Hong, Steven [3 ]
Jin, Yong-Ming [4 ]
Rubin, Allan M. [5 ]
机构
[1] Univ Toledo, Dept Surg, Div Otolaryngol & Dent, Coll Med, Toledo, OH 43614 USA
[2] Renovo Neural Inc, Cleveland, OH USA
[3] ATTN MCHK DSH, Tripler Army Med Ctr, Tripler, HI USA
[4] Cleveland Clin, Cerebrovasc Ctr, Cleveland, OH 44106 USA
[5] NW Ohio ENT Consulatants, Sylvania, OH USA
来源
JOURNAL OF VESTIBULAR RESEARCH-EQUILIBRIUM & ORIENTATION | 2011年 / 21卷 / 04期
关键词
GABA; taurine; glycine; glutamate; aspartate; GAMMA-AMINOBUTYRIC-ACID; CEREBELLAR PEDUNCLE TRANSECTION; PURKINJE-CELL TERMINALS; D-ASPARTATE RECEPTORS; COMMISSURAL NEURONS; COCHLEAR NUCLEUS; GLUTAMATE-DECARBOXYLASE; IMMUNOCYTOCHEMICAL LOCALIZATION; FASTIGIOVESTIBULAR PROJECTIONS; UNILATERAL DEAFFERENTATION;
D O I
10.3233/VES-2011-0419
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Changes in concentrations of amino acids, especially GABA, glutamate, and aspartate, occur in vestibular nuclei after removal of cerebellar and labyrinth inputs. Here, we examined the effects of transecting midline-crossing connections between the two vestibular nuclear complexes, which especially include commissural connections. Three rats were euthanized at each of 2, 7, and 30 days after a midline cut at the level of the vestibular nuclei. Two sham-lesioned rats were prepared for surgery but no cut made. Samples of superior (SuVN), dorsal and ventral lateral (LVNd and LVNv), dorsal and ventral medial (MVNd and MVNv), and spinal vestibular nuclei (SpVN) were microdissected from freeze-dried coronal sections and assayed for amino acid concentrations. Reductions of GABA concentration occurred by 2 days and continued through 30 days after surgery in most regions. Glutamate and aspartate concentrations decreased by 2 days in LVN and MVN, then glutamate showed some recovery by 30 days. Glutamine and taurine concentrations increased in almost all regions. Glycine concentration decreased in MVN and LVNv. Our results support association of GABA, glutamate, aspartate, and to some extent glycine, with vestibular crossed connections. Comparisons to our previous studies suggest some complex lesion effects, especially in LVNd.
引用
收藏
页码:175 / 191
页数:17
相关论文
共 96 条
[51]  
KNOPFEL T, 1988, EXP BRAIN RES, V72, P129
[52]   IMMUNOHISTOCHEMICAL LOCALIZATION OF GAMMA-AMINOBUTYRIC ACID-CONTAINING AND ASPARTATE-CONTAINING NEURONS IN THE RAT DEEP CEREBELLAR NUCLEI [J].
KUMOI, K ;
SAITO, N ;
KUNO, T ;
TANAKA, C .
BRAIN RESEARCH, 1988, 439 (1-2) :302-310
[53]   PRIMARY AFFERENT EXCITATORY TRANSMISSION RECORDED INTRACELLULARLY INVITRO FROM RAT MEDIAL VESTIBULAR NEURONS [J].
LEWIS, MR ;
PHELAN, KD ;
SHINNICKGALLAGHER, P ;
GALLAGHER, JP .
SYNAPSE, 1989, 3 (02) :149-153
[54]   Quantitative autoradiography of 5-[H-3]6-cyano-7-nitro-quinoxaline-2,3-dione and (+)-3-[H-3]dizocilpine maleate binding in rat vestibular nuclear complex after unilateral deafferentation, with comparison to cochlear nucleus [J].
Li, H ;
Godfrey, DA ;
Rubin, AM .
NEUROSCIENCE, 1997, 77 (02) :473-484
[55]   Immunohistochemical study on the distributions of AMPA receptor subtypes in rat vestibular nuclear complex after unilateral deafferentation [J].
Li, HY ;
Godfrey, TG ;
Godfrey, DA ;
Rubin, AM .
NEW DIRECTIONS IN VESTIBULAR RESEARCH, 1996, 781 :653-655
[56]  
Li HY, 1996, J NEUROCHEM, V66, P1550
[57]   COMPARISON OF SURGERIES FOR REMOVAL OF PRIMARY VESTIBULAR INPUTS - A COMBINED ANATOMICAL AND BEHAVIORAL-STUDY IN RATS [J].
LI, HY ;
GODFREY, DA ;
RUBIN, AM .
LARYNGOSCOPE, 1995, 105 (04) :417-424
[58]  
LOWRY OH, 1972, FLEXIBLE SYSTEM ENZY, P221
[59]   Functional Organization of Vestibular Commissural Connections in Frog [J].
Malinvaud, David ;
Vassias, Isabelle ;
Reichenberger, Ingrid ;
Roessert, Christian ;
Straka, Hans .
JOURNAL OF NEUROSCIENCE, 2010, 30 (09) :3310-3325
[60]  
MANO N., 1968, BRAIN RES, V8, P378, DOI 10.1016/0006-8993(68)90058-9