Discrimination of Korean cattle (Hanwoo) using DNA markers derived from SNPs in bovine mitochondrial and SRY genes

被引：6

作者：

Yoon, D. ^{[2
]}

Kwon, Y. S. ^{[1
]}

Lee, K. Y. ^{[1
]}

Jung, W. Y. ^{[1
]}

Sasazaki, S. ^{[3
]}

Mannen, H. ^{[3
]}

Jeon, J. T. ^{[4
]}

Lee, J. H. ^{[1
]}

机构：

[1] Chungnam Natl Univ, Res Ctr Trangen Cloned Pigs, Div Anim Sci & Resources, Taejon 305764, South Korea

[2] Natl Inst Anim Sci, Div Anim Genom & Bioinformat, Suwon 441350, South Korea

[3] Kobe Univ, Grad Sch Agr Sci, Kobe, Hyogo 6578501, Japan

[4] Gyeongsang Natl Univ, Div Appl Life Sci, Jinju 660701, South Korea

来源：

ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES | 2008年 / 21卷 / 01期

关键词：

Hanwoo; Bos taurus; Bos indicus; PCR-RFLP; SRY; ND4; ND5;

D O I：

10.5713/ajas.2008.70209

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

In order to distinguish Korean cattle (Hanwoo) beef from the imported beef from Australia in Korean markets, DNA markers based on PCR-RFLP from mitochondrial genes and SRY gene were applied. A total of 2,826 beef samples comprising 1,495 Hanwoo and 1,331 foreign cattle breeds were obtained in Korea. An 801 bp fragment of the SRY gene on the bovine Y chromosome, a 343 bp fragment of ND4 gene and a 528 bp fragment of ND5 gene in the bovine mtDNA were amplified by PCR and digested with three restriction enzymes, Msel, HpyCH(4)III and Tsp509I, respectively. The results showed that Bos taurus (T) type was the majority in Hanwoo by combining three markers (99.5%). However, 78.2% of Bos indicus (1) type was observed in the imported beef samples. These results indicated that three markers used in this study will be used as valuable markers for discriminating imported beef against Hanwoo.

引用

页码：25 / 28

页数：4

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