CHIRAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF ENANTIOMERS OF THREO-LOSIGAMONE

被引：2

作者：

Zhou, Jie ^{[1
,2
]}

Liu, Qian ^{[1
]}

Li, Yinghui ^{[1
]}

Zhang, Zhenzhong ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Sch Pharm, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Res Ctr Nanotechnol Drug, Zhengzhou 450001, Henan, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 09期

关键词：

chiralpak IB; chiral stationary phase; enantiomeric separation; HPLC; losigamone; threo-losigamone; EPILEPTIFORM ACTIVITY; HIPPOCAMPAL-NEURONS; ANTIEPILEPTIC DRUG; STATIONARY-PHASE; CELLULOSE; SLICES; DERIVATIVES; DECREASES; CORTEX;

D O I：

10.1080/10826076.2011.619033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of threo-losigamone. The enantiomers were separated by a Chiralpak IB (250 x 4.6 mm, mm, 5 mu m) at 30 degrees C and detected at 230 nm; the mobile phase used was n-hexane-ethanol (90/10, V/V) with a flow rate of 0.8 mL . min(-1). The resolution of threo-losigamone enantiomers was 1.79. By comparing the chromatographs of losigamone enantiomers and losigamone spiked with threo-losigamone, it was determined that the first effluent was threo-losigamone.

引用

页码：1238 / 1244

页数：7

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