CHIRAL HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF ENANTIOMERS OF THREO-LOSIGAMONE

被引：2

作者：

Zhou, Jie ^{[1
,2
]}

Liu, Qian ^{[1
]}

Li, Yinghui ^{[1
]}

Zhang, Zhenzhong ^{[1
,2
]}

机构：

[1] Zhengzhou Univ, Sch Pharm, Zhengzhou 450001, Henan, Peoples R China

[2] Zhengzhou Univ, Res Ctr Nanotechnol Drug, Zhengzhou 450001, Henan, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 09期

关键词：

chiralpak IB; chiral stationary phase; enantiomeric separation; HPLC; losigamone; threo-losigamone; EPILEPTIFORM ACTIVITY; HIPPOCAMPAL-NEURONS; ANTIEPILEPTIC DRUG; STATIONARY-PHASE; CELLULOSE; SLICES; DERIVATIVES; DECREASES; CORTEX;

D O I：

10.1080/10826076.2011.619033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new liquid chromatographic method has been developed for the chiral separation of the enantiomers of threo-losigamone. The enantiomers were separated by a Chiralpak IB (250 x 4.6 mm, mm, 5 mu m) at 30 degrees C and detected at 230 nm; the mobile phase used was n-hexane-ethanol (90/10, V/V) with a flow rate of 0.8 mL . min(-1). The resolution of threo-losigamone enantiomers was 1.79. By comparing the chromatographs of losigamone enantiomers and losigamone spiked with threo-losigamone, it was determined that the first effluent was threo-losigamone.

引用

页码：1238 / 1244

页数：7

共 16 条

[1] Efficacy and safety of Losigamone in partial seizures: a randomized double-blind study [J].

Baulac, M ;

Klement, S .

EPILEPSY RESEARCH, 2003, 55 (03) :177-189

[2] Progress report on new antiepileptic drugs: a summary of the fourth Eilat conference (EILAT IV) [J].

Bialer, M ;

Johannessen, SI ;

Kupferberg, HJ ;

Levy, RH ;

Loiseau, P ;

Perucca, E .

EPILEPSY RESEARCH, 1999, 34 (01) :1-41

[3]

Borowicz K. K., 2003, NEUROL NEUROCHIR POL, V3, P35

[4] Losigamone decreases spontaneous synaptic activity in cultured hippocampal neurons [J].

Draguhn, A ;

Jungclaus, M ;

Sokolowa, S ;

Heinemann, U .

EUROPEAN JOURNAL OF PHARMACOLOGY, 1997, 325 (2-3) :245-251

[5] The antiepileptic drug losigamone decreases the persistent Na+ current in rat hippocampal neurons [J].

Gebhardt, C ;

Breustedt, JM ;

Nöldner, M ;

Chatterjee, SS ;

Heinemann, U .

BRAIN RESEARCH, 2001, 920 (1-2) :27-31

[6] EFFECTS OF THE TETRONIC ACID-DERIVATIVES AO33 (LOSIGAMONE) AND AO78 ON EPILEPTIFORM ACTIVITY AND ON STIMULUS-INDUCED CALCIUM-CONCENTRATION CHANGES IN RAT HIPPOCAMPAL SLICES [J].

KOHR, G ;

HEINEMANN, U .

EPILEPSY RESEARCH, 1990, 7 (01) :49-58

[7]

Liu G. Z., 2010, SYNTHESIS BIOACTIVIT

[8] Pharmacokinetics of [14C]-labelled Losigamone and enantiomers after oral administration to healthy subjects [J].

Peeters, PAM ;

Van Lier, JJ ;

Van De Merbel, N ;

Oosterhuis, B ;

Wieling, J ;

Jonkman, JHG ;

Klessing, K ;

Biber, A .

EUROPEAN JOURNAL OF DRUG METABOLISM AND PHARMACOKINETICS, 1998, 23 (01) :45-53

[9] Reversed-phase chiral HPLC and LC/MS analysis with tris(chloromethylphenylcarbamate) derivatives of cellulose and amylose as chiral stationary phases [J].

Peng, Liming ;

Jayapalan, Swapna ;

Chankvetadze, Bezhan ;

Farkas, Tivadar .

JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (44) :6942-6955

[10] EFFECTS OF LOSIGAMONE ON SYNAPTIC POTENTIALS AND SPIKE FREQUENCY HABITUATION IN RAT ENTORHINAL CORTEX AND HIPPOCAMPAL CA1 NEURONS [J].

SCHMITZ, D ;

GLOVELI, T ;

HEINEMANN, U .

NEUROSCIENCE LETTERS, 1995, 200 (02) :141-143

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