Glucose biosensor based on carbon/PVC-COOH/ferrocene composite with covalently immobilized enzyme

被引:0
|
作者
Li, CX [1 ]
Zeng, YL [1 ]
Tang, CR [1 ]
机构
[1] Hunan Univ Sci & Technol, Chem & Chem Engn Coll, Xiangtan 411201, Peoples R China
关键词
PVC-COOH; ferrocene; glucose oxidase; glucose;
D O I
暂无
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A carbon/PVC-COOH/ferrocene composite electrode used for the determination of glucose has been prepared. The ferrocene acted as mediator was incorporated into the PVC-COOH polymer and the leakage could be prevented. The presence of carboxyl groups on the electrode surface allowed immobilizing enzyme via EDC and NHS. The ratio of PVC-COOH to graphite powder (w/w) has been studied. Amperometric determination of glucose has been performed at potential of 0.30 V vs SCE. The response time was < 15 s. The linear response range was of 0.1-20 mmol/L with a detection limit of 48 mu mol/L.
引用
收藏
页码:1357 / 1360
页数:4
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