Structure elucidation of an immunoenhancing pectic polysaccharide isolated from aqueous extract of pods of green bean (Phaseolus vulgaris L.)

被引：50

作者：

Patra, Pradip ^{[1
]}

Das, Debsankar ^{[1
]}

Behera, Birendra ^{[2
]}

Maiti, Tapas K. ^{[2
]}

Islam, Syed S. ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, W Bengal, India

[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, W Bengal, India

来源：

CARBOHYDRATE POLYMERS | 2012年 / 87卷 / 03期

关键词：

Phaseolus vulgaris L; Pectin; Structure; NMR spectroscopy; Immunoenhancing activity; Antioxidant activity; PANAX-GINSENG; ACIDIC POLYSACCHARIDE; HYPOGLYCEMIC ACTIVITY; ANTIDIABETES DRUGS; OLIGOSACCHARIDES; CONFIGURATION; ANTITUMOR; GLYCANS;

D O I：

10.1016/j.carbpol.2011.10.042

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A water-soluble pectic polysaccharide (PS-I) isolated from the hot water extract of the pods of green bean (Phaseolus vulgaris L.) by successive purification through dialysis, centrifugation and Sepharose-6B gel filtration was found to consist of methyl ester of D-galacturonic acid, D-galactose, and L-arabinose in a molar ratio of nearly 2:2:1. This molecule showed splenocyte, thymocyte activation as well as antioxidant properties. Structural studies of PS-I was performed using acid hydrolysis, methylation analysis, periodate oxidation studies along with NMR analysis (H-1, C-13, TOCSY, DQF-COSY, NOESY, ROESY, HMQC, and HMBC) and the repeating unit of PS-I was established as: -> 4)-alpha-D-GalpA6Me-(1 -> 4)-alpha-D-GalpA6Me-(1 -> 2 up arrow I alpha-D-Galp-(1 -> 3)-beta-L-Arap-(1 -> 4)-beta-D-Galp (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2169 / 2175

页数：7

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