A tudor domain protein, SIMR-1, promotes siRNA production at piRNA-targeted mRNAs in C. elegans

被引：44

作者：

Manage, Kevin, I ^{[1
]}

Rogers, Alicia K. ^{[1
]}

Wallis, Dylan C. ^{[1
]}

Liebel, Celja J. ^{[1
]}

Anderson, Dorian C. ^{[1
]}

Nguyen, Dieu An H. ^{[1
]}

Arca, Katerina ^{[1
]}

Brown, Kristen C. ^{[2
,3
]}

Rodrigues, Ricardo J. Cordeiro ^{[4
,5
]}

de Albuquerque, Bruno F. M. ^{[4
]}

Ketting, Rene F. ^{[4
]}

Montgomery, Taiowa A. ^{[2
]}

Phillips, Carolyn Marie ^{[1
]}

机构：

[1] Univ Southern Calif, Dept Biol Sci, Los Angeles, CA 90007 USA

[2] Colorado State Univ, Dept Biol, Ft Collins, CO 80523 USA

[3] Colorado State Univ, Cell & Mol Biol Program, Ft Collins, CO 80523 USA

[4] Inst Mol Biol, Biol Noncoding RNA Grp, Mainz, Germany

[5] Int PhD Programme Gene Regulat Epigenet & Genome, Mainz, Germany

来源：

ELIFE | 2020年 / 9卷

基金：

美国国家科学基金会;

关键词：

GERMLINE GENE-EXPRESSION; ARGININE METHYLATION; PIWI PROTEINS; STRUCTURAL BASIS; TRANSPOSABLE ELEMENT; ARGONAUTE PROTEINS; EPIGENETIC MEMORY; CELL IMMORTALITY; GENOME; FAMILY;

D O I：

10.7554/eLife.56731

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

piRNAs play a critical role in the regulation of transposons and other germline genes. In Caenorhabditis elegans, regulation of piRNA target genes is mediated by the mutator complex, which synthesizes high levels of siRNAs through the activity of an RNA-dependent RNA polymerase. However, the steps between mRNA recognition by the piRNA pathway and siRNA amplification by the mutator complex are unknown. Here, we identify the Tudor domain protein, SIMR-1, as acting downstream of piRNA production and upstream of mutator complex-dependent siRNA biogenesis. Interestingly, SIMR-1 also localizes to distinct subcellular foci adjacent to P granules and Mutator foci, two phase-separated condensates that are the sites of piRNA-dependent mRNA recognition and mutator complex-dependent siRNA amplification, respectively. Thus, our data suggests a role for multiple perinuclear condensates in organizing the piRNA pathway and promoting mRNA regulation by the mutator complex.

引用

页数：33

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