Generation of human induced pluripotent stem cell- derived cerebral organoids for cellular and molecular characterization

被引：6

作者：

Anastasaki, Corina ^{[1
]}

Wilson, Anna F. ^{[1
]}

Chen, Alexander S. ^{[1
]}

Wegscheid, Michelle L. ^{[1
]}

Gutmann, David H. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Neurol, St Louis, MO 63110 USA

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 01期

关键词：

NEURONS;

D O I：

10.1016/j.xpro.2022.101173

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human induced pluripotent stem cell (hiPSC)-derived cerebral organoids (COs) can serve as an in vitro model for studying normal and pathologic human brain development. Here, we optimized existing protocols to streamline the genera-tion of forebrain COs from hiPSCs. We employ these COs to define the impact of disease-causing mutations on cell fate, differentiation, maturation, and morphology relevant to neurodevelopmental disorders. Although limited to forebrain CO identity, this schema requires minimal external interference and is amenable to low-throughput biochemical assays.For complete details on the use and execution of this profile, please refer to Anastasaki et al. (2020) and Wegscheid et al. (2021).

引用

页数：21

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