Srv2 Is a Pro-fission Factor that Modulates Yeast Mitochondrial Morphology and Respiration by Regulating Actin Assembly

被引：20

作者：

Chen, Ying-Chieh ^{[1
]}

Cheng, Tzu-Hao ^{[2
]}

Lin, Wei-Ling ^{[1
]}

Chen, Chang-Lin ^{[1
]}

Yang, Wei Yuan ^{[3
]}

Blackstone, Craig ^{[4
]}

Chang, Chuang-Rung ^{[1
]}

机构：

[1] Natl Tsing Hua Univ, Inst Biotechnol, Bldg 2,Room 506 101,Sect 2,Kuang Fu Rd, Hsinchu 30013, Taiwan

[2] Natl Yang Ming Univ, Inst Biochem & Mol Biol, Taipei 11221, Taiwan

[3] Acad Sinica, Inst Biol Chem, Taipei 11529, Taiwan

[4] NINDS, Cell Biol Sect, Neurogenet Branch, NIH, Bldg 35,Room 2A 201,9000 Rockville Pike, Bethesda, MD 20892 USA

来源：

ISCIENCE | 2019年 / 11卷

关键词：

SACCHAROMYCES-CEREVISIAE; PROFILIN; GTPASE; FUSION; CYCLASE; DNM1P; COMPONENT; MITOPHAGY; TURNOVER; CONSTRICTION;

D O I：

10.1016/j.isci.2018.12.021

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Dynamic processes such as fusion, fission, and trafficking are important in the regulation of cellular organelles, with an abundant literature focused on mitochondria. Mitochondria' dynamics not only help shape its network within cells but also are involved in the modulation of respiration and integrity. Disruptions of mitochondria' dynamics are associated with neurodegenerative disorders. Although proteins that directly bind mitochondria to promote membrane fusion/fission have been studied intensively, machineries that regulate dynamic mitochondria' processes remain to be explored. We have identified an interaction between the mitochondrial fission GTPase Dnm1/DRP1 and the actin-regulatory protein Srv2/CAP at mitochondria. Deletion of Srv2 causes elongated-hyperfused mitochondria and reduces the reserved respiration capacity in yeast cells. Our results further demonstrate that the irregular network morphology in Jsrv2 cells derives from disrupted actin assembly at mitochondria. We suggest that Srv2 functions as a pro-fission factor in shaping mitochondrial dynamics and regulating activity through its actin-regulatory effects.

引用

页码：305 / +

页数：31

共 63 条

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