Electrochemical Quantification of Escherichia coli with DNA Nanostructure

被引：75

作者：

Giovanni, Marcella ^{[1
]}

Setyawati, Magdiel Inggrid ^{[1
]}

Tay, Chor Yong ^{[1
]}

Qian, Hang ^{[1
]}

Kuan, Win Sen ^{[2
]}

Leong, David Tai ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Chem & Biomol Engn, Singapore 117585, Singapore

[2] Natl Univ Singapore, Natl Univ Hosp, Natl Univ Hlth Syst, Dept Surg,Yong Loo Lin Sch Med,Emergency Med Dept, Singapore 119074, Singapore

来源：

ADVANCED FUNCTIONAL MATERIALS | 2015年 / 25卷 / 25期

关键词：

biosensor; DNA nanostructures; Escherichia coli; BIOSENSORS; DELIVERY;

D O I：

10.1002/adfm.201500940

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Escherichia coli is an indicator of fecal contamination in recreational water and some strains are responsible for serious food poisoning in humans. Therefore, having a rapid, sensitive, selective, and simple E. coli detection is important. In this study, DNA nanopyramids anchor is utilized for immunological detection of E. coli lipopolysaccharides, lysate, and whole bacteria. By measuring the E. coli amount electrochemically, detection limits of 0.20 ng mL(-1)E. coli lipopolysaccharides and 1.20 CFU mL(-1) equivalent of lysed E. coli bacteria are obtained. Our method confers selectivity to detect E. coli in the presence of various other macromolecules. Therefore, our system can be developed to test the E. coli content in real water samples.

引用

页码：3840 / 3846

页数：7

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