Diversity of chromosomal AmpC β-lactamases from Enterobacter cloacae isolates in a Portuguese hospital

被引：12
作者：
Conceiçào, T
Faria, N
Lito, L
Cristino, JM
Salgado, MJ
Duarte, A
机构：
[1] Fac Pharm, P-1619049 Lisbon, Portugal
[2] Hosp Santa Maria, Fac Med, Lisbon, Portugal
来源：
FEMS MICROBIOLOGY LETTERS | 2004年 / 230卷 / 02期
关键词：
Enterobacter cloacae; beta-lactamase; AmpC;
D O I：
10.1016/S0378-1097(03)00891-7
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Six clinical isolates of Enterobacter cloacae isolated in a Portuguese hospital, between April 1999 and November 2000, demonstrated resistance to almost all broad-spectrum cephalosporins, except to cefepime. These isolates were susceptible to quinolones and to aminoglycosides. lsoelectric focusing demonstrated production of beta-lactamases with pIs > 8.0 and by all six isolates, exhibiting a cephalosporinase phenotype. The results of pulsed field gel electrophoresis revealed that these isolates were genetically unrelated. The amino acid sequence of six AmpC beta-lactamases (EclolFF, Eclo6FF, Eclo9FF, Eclo1OFF, Eclo11FF and Eclol5FF) shared 97-99% homology with the chromosomal AmpC beta-lactamase from E. cloacae P99 and 86-87% homology with those of two plasmid-mediated AmpC beta-lactamases, MIR-1 and ACT-1. This is the first report of chromosomal AmpC beta-lactamase production by E cloacae isolates in a Portuguese hospital. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
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页码：197 / 202
页数：6
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