Mechanism of microwave-accelerated soy protein isolate-saccharide graft reactions

被引：61

作者：

Guan, Jun-Jun ^{[1
]}

Zhang, Tong-Bin ^{[2
]}

Hui, Ming ^{[1
]}

Yin, Hai-Cheng ^{[1
]}

Qiu, Ai-Yong ^{[3
]}

Liu, Xiao-Ya ^{[4
]}

机构：

[1] Henan Univ Technol, Coll Bioengn, Zhengzhou 450001, Peoples R China

[2] Henan Univ Technol, Coll Sci, Zhengzhou 450001, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[4] Jiangnan Univ, Sch Chem & Mat Engn, Wuxi 214122, Peoples R China

来源：

FOOD RESEARCH INTERNATIONAL | 2011年 / 44卷 / 09期

关键词：

Microwave; Soy protein isolate; Saccharide; Graft reactions; Mechanism; ORGANIC-SYNTHESIS; FOOD PROTEINS; AGGREGATION; BINDING;

D O I：

10.1016/j.foodres.2011.05.015

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

To investigate the mechanism of microwave-accelerated soy protein isolate (SPI)-saccharide graft reactions, we examined effects of microwave heating (MH) and water-bath heating (WH) on protein structures and reaction properties. Under high-power microwave irradiation, protein disulfide bonds were broken to cause an increase first and a decrease later in free sulfhydryl contents over time, thus inducing subunit disaggregation. And the solvent (water) exposure of hydrophobic core residues was enhanced in MH-treated SPI, which led to protein disaggregation and unfolding and much smaller aggregates in the protein solution. Calculations showed that graft reactions by MH had much lower activation energies than those by WH. Moreover, microwave can reduce the occurrence of caramelization and thus improve the graft reaction selectivity. It is seen that the microwave radiation power strongly influenced the reaction kinetics. Consequently, the results suggest that improvement of microwave-assisted SPI-saccharide graft reactions is mainly due to a thermal microwave effect and therefore the reaction mechanism could be reasonably inferred. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2647 / 2654

页数：8

共 31 条

[21]

LIU HX, 1997, SCI CHINA, V27, P350

[22] Molecular and structural analysis of electrophoretic variants of soybean seed storage proteins [J].

Maruyama, N ;

Fukuda, T ;

Saka, S ;

Inui, N ;

Kotoh, J ;

Miyagawa, M ;

Hayashi, M ;

Sawada, M ;

Moriyama, T ;

Utsumi, S .

PHYTOCHEMISTRY, 2003, 64 (03) :701-708

[23]

Miklavc A, 2001, CHEMPHYSCHEM, V2, P552, DOI 10.1002/1439-7641(20010917)2:8/9<552::AID-CPHC552>3.0.CO

[24]

2-5

[25] Microwave Chemistry in Silicon Carbide Reaction Vials: Separating Thermal from Nonthermal Effects [J].

Obermayer, David ;

Gutmann, Bernhard ;

Kappe, C. Oliver .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (44) :8321-8324

[26] Non-thermal effects of microwaves on proteins: Thermophilic enzymes as model system [J].

Porcelli, M ;

Cacciapuoti, G ;

Fusco, S ;

Massa, R ;

dAmbrosio, G ;

Bertoldo, C ;

DeRosa, M ;

Zappia, V .

FEBS LETTERS, 1997, 402 (2-3) :102-106

[27] SDS-resistant aggregation of membrane proteins: Application to the purification of the vesicular monoamine transporter [J].

Sagne, C ;

Isambert, MF ;

Henry, JP ;

Gasnier, B .

BIOCHEMICAL JOURNAL, 1996, 316 :825-831

[28] USE OF NONREDUCING SDS-PAGE FOR MONITORING RENATURATION OF RECOMBINANT PROTEIN-SYNTHESIS INITIATION-FACTOR, EIF-4-ALPHA [J].

STERN, BD ;

WILSON, M ;

JAGUS, R .

PROTEIN EXPRESSION AND PURIFICATION, 1993, 4 (04) :320-327

[29] Diffusion in microwave-heated ceramics [J].

Whittaker, AG .

CHEMISTRY OF MATERIALS, 2005, 17 (13) :3426-3432

[30] Effects of microwave dielectric heating on heterogeneous catalysis [J].

Zhang, XL ;

Hayward, DO ;

Mingos, DMP .

CATALYSIS LETTERS, 2003, 88 (1-2) :33-38

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