Pellet feed adsorbed with the recombinant Lactococcus lactis BFE920 expressing SiMA antigen induced strong recall vaccine effects against Streptococcus iniae infection in olive flounder (Paralichthys olivaceus)

被引:25
作者
Kim, Daniel [1 ]
Beck, Bo Ram [1 ]
Lee, Sun Min [2 ]
Jeon, Jongsu [3 ]
Lee, Dong Wook [4 ]
Lee, Jae Il [5 ]
Song, Seong Kyu [1 ]
机构
[1] Handong Univ, Sch Life Sci, Pohang 791708, South Korea
[2] Seoul Natl Univ, Bundang Hosp, Dept Internal Med, Songnam, Gyeonggi Do, South Korea
[3] Medytox Inc, New Business Dev Dept, Seoul, South Korea
[4] Pohang Univ Sci & Technol, Div Integrat Biosci & Biotechnol, Pohang 790784, South Korea
[5] GyeongSangbuk Do Fisheries Technol Ctr, Pohang 791941, South Korea
基金
新加坡国家研究基金会;
关键词
Olive flounder; Lactococcus lactis BFE920; Feed vaccine; Oral immunization; Vaccine vehicle; Streptococcus iniae; Streptococcosis; ORAL TOLERANCE; IMMUNE-RESPONSES; INNATE IMMUNITY; IFN-GAMMA; FISH; PROTEIN; RESISTANCE; PROBIOTICS; INDUCTION; CYTOKINE;
D O I
10.1016/j.fsi.2016.06.010
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The aim of this study was to develop a fish feed vaccine that provides effective disease prevention and convenient application. A lactic acid bacterium (LAB), Lactococcus lactis BFE920, was modified to express the SiMA antigen, a membrane protein of Streptococcus iniae. The antigen was engineered to be expressed under the nisin promoter, which is induced by nisin produced naturally by the host LAB. Various sizes (40 +/- 3.5 g, 80 +/- 2.1 g, and 221 +/- 2.4 g) of olive flounder (Paralichthys olivaceus) were vaccinated by feeding the extruded pellet feed, onto which the SiMA-expressing L. lactis BFE920 (1.0 x 10(7) CFU/g) was adsorbed. Vaccine-treated feed was administered twice a day for 1 week, and priming and boosting were performed with a 1-week interval in between. The vaccinated fish had significantly elevated levels of antigen-specific serum antibodies and T cell marker mRNAs: CD4-1, CD4-2, and CD8a. In addition, the feed vaccine significantly induced T cell effector functions, such as the production of IFN-gamma and activation of the transcription factor that induces its expression, T-bet. When the flounder were challenged by intraperitoneal infection and bath immersion with S. iniae, the vaccinated fish showed 84% and 82% relative percent survival (RPS), respectively. Furthermore, similar protective effects were confirmed even 3 months after vaccination in a field study (n = 4800), indicating that this feed vaccine elicited prolonged duration of immunopotency. In addition, the vaccinated flounder gained 21% more weight and required 16% less feed to gain a unit of body weight compared to the control group. The data clearly demonstrate that the L. lactis BFE920-SiMA feed vaccine has strong protective effects, induces prolonged vaccine efficacy, and has probiotic effects. In addition, this LAB-based fish feed vaccine can be easily used to target many different pathogens of diverse fish species. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码:374 / 383
页数:10
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