Rituximab (anti-CD20)-modified AZD-2014-encapsulated nanoparticles killing of B lymphoma cells

被引:9
作者
Tang, Xiaolong [1 ]
Xie, Chunmei [2 ]
Jiang, Zhenyou [3 ,4 ]
Li, Amin [1 ]
Cai, Shiyu [1 ]
Hou, Changhao [1 ]
Wang, Jian [1 ]
Liang, Yong [5 ,6 ]
Ma, Dong [7 ]
机构
[1] Anhui Univ Sci & Technol, Med Coll, Huainan, Peoples R China
[2] Guangzhou Med Univ, Guangzhou Peoples Hosp 8, Dept Lab Med, Guangzhou, Guangdong, Peoples R China
[3] Jinan Univ, Dept Microbiol, Guangzhou, Guangdong, Peoples R China
[4] Jinan Univ, Dept Immunol, Guangzhou, Guangdong, Peoples R China
[5] Xuzhou Med Coll, Huaian Hosp, Huaian, Peoples R China
[6] Huaian Second Hosp, Huaian, Peoples R China
[7] Jinan Univ, Dept Biomed Engn, Guangdong Higher Educ Inst, Key Lab Biomat, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
Non-Hodgkin's lymphoma; mTOR; signal pathways; rituximab; nanoparticles; TOR serine-threonine kinases; AZD-2014; NON-HODGKINS-LYMPHOMA; PRECLINICAL EVALUATION; INHIBITOR; MTOR; METABOLISM; ACTIVATION; INITIATION; APOPTOSIS; PATHWAYS; GROWTH;
D O I
10.1080/21691401.2018.1478844
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The mTOR signal pathway is often highly activated in B-cell non-Hodgkin's lymphoma (NHL) and promotes cancer progression and chemo-resistance. Therefore, the pathways of mTOR are an important target for drug development in this disease. In the current study, we developed a rituximab (anti-CD20)-modified mTOR inhibitor, AZD-2014, loaded into nanoparticles (Ab-NPs-AZD-2014) for trial of its anti-NHL effect. In a cultured NHL cell line, Ab-NPs-AZD-2014 inhibited cancer cell growth, induced cell apoptosis, and blocked activation of mTORC1 and mTORC2 in Raji cells. These results indicate that antibody modification and nanomaterial loading of AZD-2014 with anti-CD20 significantly improved efficacy of AZD-2014 against NHL cells. This approach may ultimately deserve testing in therapy against NHL.
引用
收藏
页码:1063 / 1073
页数:11
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