Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian blue-modified gold electrode

被引：26

作者：

Yin, Bing ^{[1
]}

Yuan, Ruo ^{[1
]}

Chai, Yaqing ^{[1
]}

Chen, Shihong ^{[1
]}

Cao, Shurui ^{[1
]}

Xu, Yang ^{[1
]}

Fu, Ping ^{[1
]}

机构：

[1] SW Univ, Coll Chem & Engn, Chongqing Key Lab Analyt Chem, Chongqing 400715, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2008年 / 30卷 / 02期

基金：

中国国家自然科学基金;

关键词：

chitosan; glucose biosensor; glucose oxidase; layer-by-layer assembly; Prussian blue;

D O I：

10.1007/s10529-007-9534-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A glucose biosensor based on layer-by-layer (LBL) self-assembling of chitosan and glucose oxidase (GOD) on a Prussian blue film was developed. First, Prussian blue was deposited on a cleaned gold electrode then chitosan and GOD were assembled alternately to construct a multilayer film. The resulting amperometric glucose biosensor exhibited a fast response time (within 10 s) and a linear calibration range from 6 mu M to 1.6 mM with a detection limit of 3.1 mu M glucose (s/n = 3). With the low operating potential, the biosensor showed little interference to the possible interferents, including ascorbic acid, acetaminophen and uric acid, indicating an excellent selectivity.

引用

页码：317 / 322

页数：6

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