A dual-amplification system for colorimetric DNA detection based on the assembly of biomolecules

被引：16

作者：

Chen, Chaohui ^{[1
]}

Ji, Xinghu ^{[2
]}

机构：

[1] Jianghan Univ, Inst Interdisciplinary Res, Wuhan 430056, Hubei, Peoples R China

[2] Wuhan Univ, Coll Chem & Mol Sci, Minist Educ, Key Lab Analyt Chem Biol & Med, Wuhan 430072, Hubei, Peoples R China

来源：

CHINESE CHEMICAL LETTERS | 2018年 / 29卷 / 08期

基金：

美国国家科学基金会;

关键词：

Dual-amplification; Colorimetric; DNA detection; Self-assembly; Biosensor; HYBRIDIZATION CHAIN-REACTION; STRAND DISPLACEMENT AMPLIFICATION; TARGET RECYCLING AMPLIFICATION; ROLLING CIRCLE AMPLIFICATION; RESONANCE ENERGY-TRANSFER; GREEN I COMPLEX; MICRORNA DETECTION; ELECTROCHEMICAL BIOSENSOR; NUCLEIC-ACIDS; GENERATION;

D O I：

10.1016/j.cclet.2017.10.013

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A dual-amplification system is reported to apply in DNA sensing via the assembly of DNA and protein. In this process, the biotinylatedcapature DNA bounded with streptavidin (SA) through the biotin-streptavidin reaction, and then the assembly of DNA and protein was triggered by the linker DNA after the target hybridized with biotinylatedcapature DNA. Sequentially, the 3,3',5,5'-tetramethylbenzidine (TMB) was oxidized by H2O2 under the catalysis of horseradish peroxidase. Based on the variation of the color and the UV-vis absorbance intensities, qualitative and quantitative DNA analyses were realized. This proposed method could detect the target DNA as low as 1.75 pmol/L and discriminate perfectly matched target DNA from the mismatch DNA. What's more, it can be expanded to detect other molecules with a reasonable design of the corresponding DNA sequences. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.

引用

页码：1287 / 1290

页数：4

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