Effects of CDP-Choline and Choline on COX Pathway in LPS-Induced Inflammatory Response in Rats

被引：8

作者：

Baris, E. ^{[1
,2
]}

Simsek, O. ^{[1
]}

Efe, H. ^{[3
]}

Oncu, S. ^{[4
]}

Gelal, A. ^{[4
]}

Hamurtekin, E. ^{[5
]}

Tosun, M. ^{[2
]}

Ozbal, S. ^{[6
]}

Yuce, Z. ^{[3
]}

Arici, M. A. ^{[4
]}

机构：

[1] Dokuz Eylul Univ, Grad Sch Hlth Sci, Izmir, Turkey

[2] Izmir Univ Econ, Dept Pharmacol, Fac Med, Izmir, Turkey

[3] Dokuz Eylul Univ, Dept Med Biol, Fac Med, Izmir, Turkey

[4] Dokuz Eylul Univ, Dept Pharmacol, Fac Med, Izmir, Turkey

[5] Eastern Mediterranean Univ, Dept Pharmacol, Fac Med, Izmir, Turkey

[6] Dokuz Eylul Univ, Med Sci, Fac Med, Dept Histol & Embryol, Izmir, Turkey

来源：

INTERNATIONAL JOURNAL OF PHARMACOLOGY | 2021年 / 17卷 / 02期

关键词：

CDP-choline; choline; lipopolysaccharide; cyclooxygenase; 2; cholinergic anti-inflammatory pathway; endotoxemia; prostaglandin levels; ANTIINFLAMMATORY PATHWAY; ORGAN INJURY; RECEPTOR; HYPOTENSION; INVOLVEMENT; ENDOTOXIN; SURVIVAL; IMPROVES; SEPSIS; MODEL;

D O I：

10.3923/ijp.2021.84.96

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Background and Objective: Cytidine-5-diphosphate-choline (CDP-choline) and choline activate the cholinergic anti-inflammatory pathway in case of inflammation. This study investigated the role of CDP-choline and choline along with the contribution of the cyclooxygenase (COX) pathway on the lipopolysaccharide (LPS)-induced endotoxemia model in rats. Materials and Methods: Endotoxemia model was induced by LPS administration. CDP-choline or choline 5 min before and 6 hrs after LPS injection. The sepsis severity, body weight changes, survival rate were evaluated. Serum prostaglandins, Tumour Necrosis Factor (TNF)-alpha, total choline levels were measured. COX-2 mRNA expression and protein levels were analyzed. Spleen tissues were evaluated histomorphological. One-way analysis of variance analysis (ANOVA) or Kruskal Wallis tests was used for statistical analysis. Results: COX-2 expressions in liver and brain tissues, serum prostaglandin E-2, 6-keto prostaglandin F-1 alpha, Thromboxane A(2) and TNF alpha levels were increased 24 hrs after LPS administration. Administrations of CDP-choline or choline were decreased COX-2 expression in the liver. Serum prostaglandin levels were decreased in the CDP-choline-treated group, whereas, only prostaglandin E-2 level was decreased in the choline-treated group. Total choline levels in serum and brain were increased after CDP-choline or choline administration. Accordingly, serum TNF alpha levels and TNF alpha expression in the liver were decreased in CDP-choline and choline-treated groups. TNF alpha expression in the brain was decreased in the choline-treated group, whereas, increased in the CDP-choline-treated group. Conclusion: CDP-choline and choline decreased LPS-induced COX-2 enzyme expression and prostaglandin levels in the periphery by increasing serum and brain total choline levels in the LPS-induced endotoxemia model in rat.

引用

页码：84 / 96

页数：13

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