Evaluation of the Rapid Polymyxin NP test and its industrial version for the detection of polymyxin-resistant Enterobacteriaceae

被引：24
|
作者：
Jayol, Aurelie [1 ,2 ,3 ]
Kieffer, Nicolas [1 ,2 ,3 ]
Poirel, Laurent [1 ,2 ,3 ]
Guerin, Francois [4 ,5 ]
Guneser, Deniz [1 ,2 ,3 ]
Cattoir, Vincent [6 ]
Nordmann, Patrice [1 ,2 ,3 ,7 ,8 ]
机构：
[1] Univ Fribourg, Dept Med, Emerging Antibiot Resistance Unit, Med & Mol Microbiol, Fribourg, Switzerland
[2] Univ Fribourg, French INSERM European Unit, LEA, IAME, Fribourg, Switzerland
[3] Natl Reference Ctr Emerging Antibiot Resistance, Fribourg, Switzerland
[4] Univ Caen Normandie, EA4655, U2RM, Equipe Antibioresistance, Caen, France
[5] CHU Caen, Serv Microbiol, Caen, France
[6] CHU Rennes, Lab Bacteriol, Rennes, France
[7] Univ Lausanne, Lausanne, Switzerland
[8] Univ Hosp Ctr, Lausanne, Switzerland
来源：
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 92卷 / 02期
基金：
瑞士国家科学基金会;
关键词：
Rapid test; Polymyxin; Enterobacteriaceae; MCR;
D O I：
10.1016/j.diagmicrobio.2018.05.006
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The commercial Rapid Polymyxin NP test was evaluated to detect colistin-resistant Enterobacteriaceae. A total of 223 enterobacterial isolates corresponding to 136 resistant (including 38 MCR-like producers), 19 heteroresistant, and 78 colistin-susceptible isolates were tested. The test was performed according to the manufacturer's instruction, and the color of the wells was read after 2 and 3 hours of incubation. The results were compared with those of the homemade Rapid Polymyxin NP test, and manual broth microdilution according to EUCAST guidelines was used as the reference method to determine the performance of the test. Excellent performance of the commercial Rapid Polymyxin NP test was found with a very major error rate, a major error rate, a sensitivity, and a specificity of 1.9%, 5.1%, 98.1%, and 94.9%, respectively. The performance of the homemade Polymyxin NP test was similar, with a slightly better value for the very major error (1.2 %). (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码：90 / 94
页数：5
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