Molecular characterization of recombinant phosphoenolpyruvate carboxylase from an extreme thermophile

被引：0
|
作者：
Nakamura, T
Izui, K
机构：
[1] Kyoto Univ, Grad Sch Sci, Sakyo Ku, Kyoto 60601, Japan
[2] Kyoto Univ, Grad Sch Agr, Sakyo Ku, Kyoto 60601, Japan
来源：
ADVANCES IN CHEMICAL CONVERSIONS FOR MITIGATING CARBON DIOXIDE | 1998年 / 114卷
关键词：
D O I：
暂无
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Phosphoenolpyruvate carboxylase (PEPC) of Thermus sp., was characterized and its mechanism of stabilization was studied by means of site-directed mutagenesis. A divergent sequence at a Gly-rich region of Thermus PEPC was revealed to contribute to the activity at high temperature but not to the tolerance to the irreversible heat inactivation.
引用
收藏
页码：605 / 608
页数：4
相关论文
共 50 条
  • [21] PURIFICATION AND CHARACTERIZATION OF A NOVEL PHOSPHOENOLPYRUVATE CARBOXYLASE FROM BANANA FRUIT
    LAW, RD
    PLAXTON, WC
    BIOCHEMICAL JOURNAL, 1995, 307 : 807 - 816
  • [22] Characterization of high and low molecular mass isoforms of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum
    Rivoal, J
    Trzos, S
    Gage, DA
    Plaxton, WC
    Turpin, DH
    PHOTOSYNTHESIS: MECHANISMS AND EFFECTS, VOLS I-V, 1998, : 3403 - 3406
  • [23] Further characterization of a high molecular mass isoform of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum
    Rivoal, J
    Gage, DA
    Plaxton, WC
    Turpin, DH
    PLANT PHYSIOLOGY, 1997, 114 (03) : 77 - 77
  • [24] CHARACTERIZATION OF ARGINASE FROM THE EXTREME THERMOPHILE BACILLUS-CALDOVELOX
    PATCHETT, ML
    DANIEL, RM
    MORGAN, HW
    BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1077 (03) : 291 - 298
  • [25] CHARACTERIZATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN A RANGE OF PARASITIC PHANEROGAMES
    RENAUDIN, S
    VIDAL, J
    LARHER, F
    ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE, 1982, 106 (03): : 229 - 237
  • [26] ISOLATION AND CHARACTERIZATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE ISOENZYMES FROM COTTON LEAF TISSUE
    MUKERJI, SK
    TING, IP
    PLANT PHYSIOLOGY, 1969, S 44 : 35 - &
  • [27] Purification and characterization of phosphoenolpyruvate carboxylase from the hyperthermophilic archaeon Methanothermus sociabilis
    Sako, Y
    Takai, K
    Uchida, A
    Ishida, Y
    FEBS LETTERS, 1996, 392 (02) : 148 - 152
  • [28] PROPERTIES OF PHOSPHOENOLPYRUVATE CARBOXYKINASE OF AN EXTREME THERMOPHILE, THERMUS-THERMOPHILUS-HB8
    YOSHIZAKI, F
    IMAHORI, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1979, 43 (02): : 397 - 399
  • [29] Characterization and expression analysis of genes encoding phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxylase kinase of Lotus japonicus, a model legume
    Nakagawa, T
    Izumi, T
    Banba, M
    Umehara, Y
    Kouchi, H
    Izui, K
    Hata, S
    MOLECULAR PLANT-MICROBE INTERACTIONS, 2003, 16 (04) : 281 - 288
  • [30] PURIFICATION AND PROPERTIES OF MOLECULAR-FORMS OF PHOSPHOENOLPYRUVATE CARBOXYLASE FROM CORN LEAVES
    GORENKOVA, LG
    ABDULLAEV, A
    NASYROV, YS
    POPOVA, LP
    BIOCHEMISTRY-MOSCOW, 1989, 54 (08) : 1109 - 1113
12345