Mechanism underlying the effect of mecamylamine on nicotinic acetylcholine receptors of rat sympathetic ganglion neurons

被引：1

作者：

Purnyn, EE ^{[1
]}

Voitenko, SV ^{[1
]}

Remizov, IN ^{[1
]}

Rikhal'sky, OV ^{[1
]}

Skok, VI ^{[1
]}

机构：

[1] Natl Acad Sci Ukraine, Bogomolets Inst Physiol, Kiev, Ukraine

来源：

NEUROPHYSIOLOGY | 2001年 / 33卷 / 01期

关键词：

mecamylamine; neuronal nicotinic acetylcholine receptor; sympathetic ganglion; ganglioblockers; acetylcholine; acetylcholine current;

D O I：

10.1023/A:1010408113230

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

On neurons of the superior cervical ganglion of 3-week-old rats, we studied the mechanism underlying the blocking effect of mecamylamine on transmembrane currents evoked by iontophoretic application of acetylcholine (ACh currents); these currents were recorded with the use of a patch-clamp technique in the whole-cell configuration. The IC50 of the above agent equaled (2.7 +/- 0.3) . 10(-10) M. The blocking effect of mecamylamine on ACh current did not depend on the membrane potential and decreased with rise in the concentration of the drug. Thus, a competitive blocking mechanism mostly underlies the above phenomenon.

引用

页码：23 / 27

页数：5

共 15 条

[1]

ANICHKOV SV, 1974, SELECTIVE EFFECTS TR

[2]

ASHER P, 1979, J PHYSL, V295, P139

[3]

DERKACH VA, 1985, DOKL AKAD NAUK SSSR+, V284, P495

[4]

FRIES FD, 1955, LANCET, V969, P977

[5] IMPROVED PATCH-CLAMP TECHNIQUES FOR HIGH-RESOLUTION CURRENT RECORDING FROM CELLS AND CELL-FREE MEMBRANE PATCHES [J].

HAMILL, OP ;

MARTY, A ;

NEHER, E ;

SAKMANN, B ;

SIGWORTH, FJ .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1981, 391 (02) :85-100

[6] ANALYSIS OF MECHANISM OF ACTION OF SOME GANGLION-BLOCKING DRUGS IN RABBIT SUPERIOR CERVICAL GANGLION [J].

LEES, GM ;

NISHI, S .

BRITISH JOURNAL OF PHARMACOLOGY, 1972, 46 (01) :78-&

[7] DIFFERENT TYPES OF BLOCKADE OF CRUSTACEAN ACETYLCHOLINE-INDUCED CURRENTS [J].

LINGLE, C .

JOURNAL OF PHYSIOLOGY-LONDON, 1983, 339 (JUN) :419-437

[8]

MOYER JH, 1955, P SOC EXP BIOL MED, V90, P402

[9] POLYMETHYLCYCLOHEXYLAMINE - NEUE, HYPOTENSIV WIRKSAME SUBSTANZEN [J].

PROTIVA, M ;

RAJSNER, M ;

TRCKA, V ;

VANECEK, M ;

VEJDELEK, ZJ .

EXPERIENTIA, 1959, 15 (02) :54-55

[10] Mecamylamine selectively blocks nicotinic receptors on vasomotor sympathetic C neurons [J].

Shen, WX ;

Horn, JP .

BRAIN RESEARCH, 1998, 788 (1-2) :118-124

← 1 2 →