A novel ultrasensitive electrochemical DNA sensor based on double tetrahedral nanostructures

被引：61

作者：

Zeng, Dongdong ^{[1
]}

Zhang, Huan ^{[1
]}

Zhu, Dan ^{[2
]}

Li, Jiang ^{[2
]}

San, Lili ^{[1
]}

Wang, Zehua ^{[1
]}

Wang, Chenguang ^{[1
]}

Wang, Yunsheng ^{[1
]}

Wang, Lihua ^{[2
]}

Zuo, Xiaolei ^{[2
]}

Mi, Xianqiang ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Adv Res Inst, Lab Syst Biol, Shanghai 201210, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Appl Phys, Div Phys Biol, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2015年 / 71卷

关键词：

Electrochemical DNA sensors; DNA tetrahedral nanostructure; SEQUENCE-SPECIFIC DETECTION; AMPLIFICATION; HYBRIDIZATION; BIOSENSOR;

D O I：

10.1016/j.bios.2015.04.065

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Electrochemical DNA (E-DNA) sensor is an important tool for detecting DNA biomarker. In this work, we have demonstrated a novel strategy of E-DNA sensor based on DNA tetrahedral nanostructures for the sensitive detection of target DNA. In our design, thiol and biotin modified DNA tetrahedral nanostructures were used as capture and report probes respectively. The biotin-tagged three dimensional DNA tetrahedral nanostructures were employed for efficient signal amplification by capturing multiple catalytic enzymes. Such improved E-DNA sensor can sensitively detect DNA target as low as 1 fM with excellent differentiation ability for even single mismatch. And a mean recovery rate of 90.57% in DNA solution extracted from human serum was obtained. We have also compared this new method of attaching catalytic enzymes with the other two typical methods: One is through biotinylated single-stranded DNA (SSDNA) and the other is through gold nanoparticles (GNPs). Results indicated that the RTSPs-based enzyme amplification system showed much better performance than the other two systems. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：434 / 438

页数：5

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