ADAMTS-13 is produced by glial cells and upregulated after spinal cord injury

被引：20

作者：

Tauchi, Ryoji ^{[1
,2
]}

Imagama, Shiro ^{[1
,2
]}

Ohgomori, Tomohiro ^{[1
]}

Natori, Takamitsu ^{[1
,3
]}

Shinjo, Ryuichi ^{[1
,2
]}

Ishiguro, Naoki ^{[2
]}

Kadomatsu, Kenji ^{[1
]}

机构：

[1] Nagoya Univ, Grad Sch Med, Dept Biochem, Showa Ku, Nagoya, Aichi 4668550, Japan

[2] Nagoya Univ, Grad Sch Med, Dept Orthopaed, Showa Ku, Nagoya, Aichi 4668550, Japan

[3] Yamanashi Gakuin Univ, Dept Hlth & Nutr, Kofu, Yamanashi 4008575, Japan

来源：

NEUROSCIENCE LETTERS | 2012年 / 517卷 / 01期

关键词：

ADAMTS-13; Astrocyte; Coagulation; Microglia; Spinal cord injury; VON-WILLEBRAND-FACTOR; THROMBOTIC THROMBOCYTOPENIC PURPURA; AXON REGENERATION; NERVOUS-SYSTEM; STELLATE CELLS; ASTROCYTES; EXPRESSION; BRAIN; RAT; ENCEPHALOMYELITIS;

D O I：

10.1016/j.neulet.2012.03.002

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

ADAMTS-13, a member of the family of disintegrins and metalloproteinases with thrombospondin motifs, is produced primarily in the liver, particularly by hepatic stellate cells. This metalloproteinase cleaves von Willebrand factor multimers and thereby regulates blood coagulation. Here, we investigated the expression of ADAMTS-13 in the central nervous system. ADAMTS-13 mRNA was expressed in cultured astrocytes and microglia but not in neurons. The protein production of ADAMTS-13 was also detected in these cultured glial cells. Furthermore, we found that the expression of ADAMTS-13 was significantly increased in the rat spinal cord after injury. Supporting the in vivo data, ADAMTS-13 protein was detected in GFAP- and CD11b-positive glial cells in injured spinal cord. Consistent with this, the proteolytic activity of ADAMTS-13 was increased after spinal cord injury. Our data suggest that ADAMTS-13 may have a critical role in the central nervous system, particularly after neuronal injuries. (c) 2012 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1 / 6

页数：6