Enzymatic, urease-mediated mineralization of gellan gum hydrogel with calcium carbonate, magnesium-enriched calcium carbonate and magnesium carbonate for bone regeneration applications

被引：29

作者：

Douglas, Timothy E. L. ^{[1
]}

Lapa, Agata ^{[2
]}

Samal, Sangram Keshari ^{[3
,4
]}

Declercq, Heidi A. ^{[5
]}

Schaubroeck, David ^{[6
,7
]}

Mendes, Ana C. ^{[8
]}

Van der Voort, Pascal ^{[9
]}

Dokupil, Agnieszka ^{[10
]}

Plis, Agnieszka ^{[10
]}

De Schamphelaere, Karel ^{[11
]}

Chronakis, Ioannis S. ^{[8
]}

Pamula, Elzbieta ^{[2
]}

Skirtach, Andre G. ^{[1
,4
]}

机构：

[1] Univ Ghent, Dept Mol Biotechnol, Ghent, Belgium

[2] AGH Univ Sci & Technol, Fac Mat Sci & Ceram, Dept Biomat, Krakow, Poland

[3] Univ Ghent, Lab Gen Biochem & Phys Pharm, Ghent, Belgium

[4] Univ Ghent, Ctr Nano & Biophoton, Ghent, Belgium

[5] Univ Ghent, Dept Basic Med Sci, Tissue Engn Grp, Ghent, Belgium

[6] IMEC, Ctr Microsyst Technol CMST, Ghent, Belgium

[7] Univ Ghent, Ghent, Belgium

[8] Tech Univ Denmark DTU, NanobioSci Res Grp, DTU Food, Lyngby, Denmark

[9] Univ Ghent, Dept Inorgan Chem, COMOC, Ghent, Belgium

[10] Inst Chem Proc Coal ICHPW, Zabrze, Poland

[11] Univ Ghent, Fac Biosci Engn, Environm Toxicol Unit, Lab Environm & Aquat Ecol,GhEnToxLab, Ghent, Belgium

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2017年 / 11卷 / 12期

关键词：

hydrogel; gellan gum; carbonate; mineralization; magnesium; composite; enzyme; CORALLINE ALGAE; HYDROMAGNESITE; BEHAVIOR; PHASE;

D O I：

10.1002/term.2273

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Mineralization of hydrogel biomaterials is considered desirable to improve their suitability as materials for bone regeneration. Calcium carbonate (CaCO3) has been successfully applied as a bone regeneration material, but hydrogel-CaCO3 composites have received less attention. Magnesium (Mg) has been used as a component of calcium phosphate biomaterials to stimulate bone-forming cell adhesion and proliferation and bone regeneration in vivo, but its effect as a component of carbonate-based biomaterials remains uninvestigated. In the present study, gellan gum (GG) hydrogels were mineralized enzymatically with CaCO3, Mg-enriched CaCO3 and magnesium carbonate to generate composite biomaterials for bone regeneration. Hydrogels loaded with the enzyme urease were mineralized by incubation in mineralization media containing urea and different ratios of calcium and magnesium ions. Increasing the magnesium concentration decreased mineral crystallinity. At low magnesium concentrations calcite was formed, while at higher concentrations magnesian calcite was formed. Hydromagnesite (Mg-5(CO3)(4)(OH)(2).4H(2)O) formed at high magnesium concentration in the absence of calcium. The amount of mineral formed and compressive strength decreased with increasing magnesium concentration in the mineralization medium. The calcium:magnesium elemental ratio in the mineral formed was higher than in the respective mineralization media. Mineralization of hydrogels with calcite or magnesian calcite promoted adhesion and growth of osteoblast-like cells. Hydrogels mineralized with hydromagnesite displayed higher cytotoxicity. In conclusion, enzymatic mineralization of GG hydrogels with CaCO3 in the form of calcite successfully reinforced hydrogels and promoted osteoblast-like cell adhesion and growth, but magnesium enrichment had no definitive positive effect. Copyright (c) 2017 John Wiley & Sons, Ltd.

引用

页码：3556 / 3566

页数：11

共 36 条

[1] INFRARED-SPECTRA OF AMORPHOUS AND CRYSTALLINE CALCIUM-CARBONATE [J].