Comparative Study of the Binding of Concanavalin A to Self-Assembled Monolayers Containing a Thiolated α-Mannoside on Flat Gold and on Nanoporous Gold

被引：23
作者：
Pandey, Binod [1 ,2 ]
Tan, Yih Horng [1 ,2 ]
Fujikawa, Kohki [1 ]
Demchenko, Alexei V. [1 ]
Stine, Keith J. [1 ,2 ]
机构：
[1] Univ Missouri, Dept Chem & Biochem, St Louis, MO 63121 USA
[2] Univ Missouri, Ctr Nanosci, St Louis, MO 63121 USA
来源：
JOURNAL OF CARBOHYDRATE CHEMISTRY | 2012年 / 31卷 / 4-6期
关键词：
Nanoporous gold; Self-assembled monolayer; 8-Mercaptooctyl alpha-D-mannopyranoside; Lectin; Concanavalin A; Electrochemical impedance spectroscopy; Atomic force microscopy; Scanning electron microscopy; SURFACE-PLASMON RESONANCE; CARBOHYDRATE-PROTEIN INTERACTIONS; LABEL-FREE DETECTION; BIOMOLECULAR INTERACTIONS; NANOENGINEERING APPROACH; ESCHERICHIA-COLI; CELL RECOGNITION; LECTINS; IMMOBILIZATION; MICROARRAYS;
D O I：
10.1080/07328303.2012.683909
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have prepared SAMs containing 8-mercaptooctyl alpha-D-mannopyranoside either as a single component or in mixed SAMs with n-octanethiol on flat gold surfaces and on nanoporous gold. Electrochemical impedance spectroscopy showed that the mixed SAMs on flat gold surfaces showed the highest Con A binding, near 1:9 solution molar ratio of thiolated alpha-mannoside to n-octanethiol, whereas those on NPG showed the highest response at 1:19 solution molar ratio of thiolated alpha-mannoside to n-octanethiol. Atomic force microscopy was employed to image the monolayers and to image the bound Con A protein. Supplemental materials are available for this article. Go to the publisher's online edition of Journal of Carbohydrate Chemistry to view the free supplemental file.
引用
收藏
页码：466 / 503
页数：38
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