A protocol for studying glucose homeostasis and islet function in mice

被引：14

作者：

Al Rijjal, Dana ^{[1
]}

Wheeler, Michael B. ^{[1
,2
]}

机构：

[1] Univ Toronto, Dept Physiol, 1 Kings Coll Circle,Med Sci Bldg Rm 3352, Toronto, ON M5S 1A8, Canada

[2] Toronto Gen Res Inst, Div Adv Diagnost Metab, Toronto, ON, Canada

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 01期

基金：

加拿大健康研究院;

关键词：

Cell Biology; Cell isolation; Metabolism; Model Organisms;

D O I：

10.1016/j.xpro.2022.101171

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose tolerance test and glucose stimulated insulin secretion are important measures to determine glucose homeostasis and islet function and assess diabetes. Here, we provide a protocol where glucose tolerance test is used to study glucose homeostasis and insulin secretion in vivo, followed by islet isolation and glucose stimulated insulin secretion to determine islet function ex vivo. This protocol enables evaluation of glucose homeostasis and islets in mice which can also be applied to rat, beta cell lines and human studies.For complete details on the use and execution of this profile, please refer to Al Rijjal et al. (2021).

引用

页数：17

共 3 条

[1] Vascepa protects against high-fat diet-induced glucose intolerance, insulin resistance, and impaired β-cell function
Al Rijjal, Dana
Liu, Ying
Lai, Mi
Song, Youchen
Danaei, Zahra
Wu, Anne
Mohan, Haneesha
Wei, Li
Schopfer, Francisco J.
Dai, Feihan F.
Wheeler, Michael B.
[J]. ISCIENCE, 2021, 24 (08)
[2] Heikkinen Sami, 2007, Curr Protoc Mol Biol, VChapter 29, DOI 10.1002/0471142727.mb29b03s77
[3] Protocol for in vivo and ex vivo assessments of glucose- stimulated insulin secretion in mouse islet f3 cells
Zhu, Yun-Xia
Zhou, Yun-Cai
Zhang, Yan
Sun, Peng
Chang, Xiao-Ai
Han, Xiao
[J]. STAR PROTOCOLS, 2021, 2 (03):

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