Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification

被引:142
作者
Tambutte, Eric [1 ]
Tambutte, Sylvie [1 ]
Segonds, Natacha [1 ]
Zoccola, Didier [1 ]
Venn, Alexander [1 ]
Erez, Jonathan [2 ]
Allemand, Denis [1 ]
机构
[1] Ctr Sci Monaco, Monaco, Monaco
[2] Hebrew Univ Jerusalem, Inst Earth Sci, IL-91904 Jerusalem, Israel
关键词
coral; biomineralization; membrane permeability; calcium transport; cell junctions; para/transcellular transport; STYLOPHORA-PISTILLATA; HERMATYPIC CORALS; SCLERACTINIAN CORAL; CALCIUM-TRANSPORT; BARRIER FUNCTION; EPITHELIUM; MECHANISMS; BIOMINERALIZATION; MACROMOLECULES; SKELETOGENESIS;
D O I
10.1098/rspb.2011.0733
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mechanisms behind the transfer of molecules from the surrounding sea water to the site of coral calcification are not well understood, but are critical for understanding how coral reefs are formed. We conducted experiments with the fluorescent dye calcein, which binds to calcium and is incorporated into growing calcium carbonate crystals, to determine the permeability properties of coral cells and tissues to this molecule, and to determine how it is incorporated into the coral skeleton. We also compared rates of calcein incorporation with rates of calcification measured by the alkalinity anomaly technique. Finally, by an electrophysiological approach, we investigated the electrical resistance of coral tissues in order to better understand the role of tissues in ionic permeability. Our results show that (i) calcein passes through coral tissues by a paracellular pathway, (ii) intercellular junctions control and restrict the diffusion of molecules, (iii) intercellular junctions should have pores of a size higher than 13 angstrom and lower than 20 nm, and (iv) the resistance of the tissues owing to paracellular junctions has a value of 477 +/- 21 Ohm cm(2). We discuss the implication of our results for the transport of calcium involved in the calcification process.
引用
收藏
页码:19 / 27
页数:9
相关论文
共 47 条
[1]   Biomineralisation in reef-building corals:: from molecular mechanisms to environmental control [J].
Allemand, D ;
Ferrier-Pagès, C ;
Furla, P ;
Houlbrèque, F ;
Puverel, S ;
Reynaud, S ;
Tambutté, É ;
Tambutté, S ;
Zoccola, D .
COMPTES RENDUS PALEVOL, 2004, 3 (6-7) :453-467
[2]   Mechanisms of carbon acquisition for endosymbiont photosynthesis in Anthozoa [J].
Allemand, D ;
Furla, P ;
Bénazet-Tambutté, S .
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 1998, 76 (06) :925-941
[3]  
Allemand D, 2011, CORAL REEFS: AN ECOSYSTEM IN TRANSITION, P119, DOI 10.1007/978-94-007-0114-4_9
[4]   CORAL SKELETONS - AN EXPLANATION OF THEIR GROWTH AND STRUCTURE [J].
BARNES, DJ .
SCIENCE, 1970, 170 (3964) :1305-&
[5]   STRUCTURE AND FORMATION OF GROWTH-RIDGES IN SCLERACTINIAN CORAL SKELETONS [J].
BARNES, DJ .
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES, 1972, 182 (1068) :331-+
[6]  
BARNES DJ, 1990, CORAL REEFS, P109
[7]   Permeability of the oral epithelial layers in cnidarians [J].
BenazetTambutte, S ;
Allemand, D ;
Jaubert, J .
MARINE BIOLOGY, 1996, 126 (01) :43-53
[8]   Paracellular pathway in the shell epithelium of Anodonta cygnea [J].
Bleher, R ;
Machado, J .
JOURNAL OF EXPERIMENTAL ZOOLOGY PART A-ECOLOGICAL AND INTEGRATIVE PHYSIOLOGY, 2004, 301A (05) :419-427
[9]  
BRAUN A, 2004, AM GEOPH U FALL M SA
[10]  
Buddemeier R.W., 1976, Oceanography and Marine Biology an Annual Review, V14, P183