An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae

被引：549

作者：

Wargacki, Adam J. ^{[1
]}

Leonard, Effendi ^{[1
]}

Win, Maung Nyan ^{[1
]}

Regitsky, Drew D. ^{[1
]}

Santos, Christine Nicole S. ^{[1
]}

Kim, Peter B. ^{[1
]}

Cooper, Susan R. ^{[1
]}

Raisner, Ryan M. ^{[1
]}

Herman, Asael ^{[1
]}

Sivitz, Alicia B. ^{[1
]}

Lakshmanaswamy, Arun ^{[1
]}

Kashiyama, Yuki ^{[1
,2
,3
]}

Baker, David ^{[4
]}

Yoshikuni, Yasuo ^{[1
]}

机构：

[1] Bio Architecture Lab, Berkeley, CA 94710 USA

[2] BAL Chile SA, Puerto Varas 5550658, Chile

[3] BAL Biofuels SA, Santiago 7550159, Chile

[4] Univ Washington, Dept Biochem, Seattle, WA 98195 USA

来源：

SCIENCE | 2012年 / 335卷 / 6066期

关键词：

TUMEFACIENS STRAIN C58; ESCHERICHIA-COLI; ALGINATE LYASE; ERWINIA-CHRYSANTHEMI; ETHANOL-PRODUCTION; MOLECULAR-IDENTIFICATION; METABOLISM; BIOSYNTHESIS; CONSTRUCTION; PURIFICATION;

D O I：

10.1126/science.1214547

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio splendidus encoding enzymes for alginate transport and metabolism. The genomic integration of this ensemble, together with an engineered system for extracellular alginate depolymerization, generated a microbial platform that can simultaneously degrade, uptake, and metabolize alginate. When further engineered for ethanol synthesis, this platform enables bioethanol production directly from macroalgae via a consolidated process, achieving a titer of 4.7% volume/volume and a yield of 0.281 weight ethanol/weight dry macroalgae (equivalent to similar to 80% of the maximum theoretical yield from the sugar composition in macroalgae).

引用

页码：308 / 313

页数：6

共 43 条

[1] Fermentation study on Saccharina latissima for bioethanol production considering variable pre-treatments [J].