An Engineered Microbial Platform for Direct Biofuel Production from Brown Macroalgae
被引:545
作者:
Wargacki, Adam J.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Wargacki, Adam J.
[1
]
Leonard, Effendi
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Leonard, Effendi
[1
]
Win, Maung Nyan
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Win, Maung Nyan
[1
]
Regitsky, Drew D.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Regitsky, Drew D.
[1
]
Santos, Christine Nicole S.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Santos, Christine Nicole S.
[1
]
Kim, Peter B.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Kim, Peter B.
[1
]
Cooper, Susan R.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Cooper, Susan R.
[1
]
Raisner, Ryan M.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Raisner, Ryan M.
[1
]
Herman, Asael
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Herman, Asael
[1
]
Sivitz, Alicia B.
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Sivitz, Alicia B.
[1
]
Lakshmanaswamy, Arun
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Lakshmanaswamy, Arun
[1
]
Kashiyama, Yuki
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USA
BAL Chile SA, Puerto Varas 5550658, Chile
BAL Biofuels SA, Santiago 7550159, ChileBio Architecture Lab, Berkeley, CA 94710 USA
Kashiyama, Yuki
[1
,2
,3
]
Baker, David
论文数: 0引用数: 0
h-index: 0
机构:
Univ Washington, Dept Biochem, Seattle, WA 98195 USABio Architecture Lab, Berkeley, CA 94710 USA
Baker, David
[4
]
Yoshikuni, Yasuo
论文数: 0引用数: 0
h-index: 0
机构:
Bio Architecture Lab, Berkeley, CA 94710 USABio Architecture Lab, Berkeley, CA 94710 USA
Yoshikuni, Yasuo
[1
]
机构:
[1] Bio Architecture Lab, Berkeley, CA 94710 USA
[2] BAL Chile SA, Puerto Varas 5550658, Chile
[3] BAL Biofuels SA, Santiago 7550159, Chile
[4] Univ Washington, Dept Biochem, Seattle, WA 98195 USA
Prospecting macroalgae (seaweeds) as feedstocks for bioconversion into biofuels and commodity chemical compounds is limited primarily by the availability of tractable microorganisms that can metabolize alginate polysaccharides. Here, we present the discovery of a 36-kilo-base pair DNA fragment from Vibrio splendidus encoding enzymes for alginate transport and metabolism. The genomic integration of this ensemble, together with an engineered system for extracellular alginate depolymerization, generated a microbial platform that can simultaneously degrade, uptake, and metabolize alginate. When further engineered for ethanol synthesis, this platform enables bioethanol production directly from macroalgae via a consolidated process, achieving a titer of 4.7% volume/volume and a yield of 0.281 weight ethanol/weight dry macroalgae (equivalent to similar to 80% of the maximum theoretical yield from the sugar composition in macroalgae).
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Atsumi, Shota
;
Hanai, Taizo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Hanai, Taizo
;
Liao, James C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, UCLA DOE Inst Genom & Proteom, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Atsumi, Shota
;
Hanai, Taizo
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Hanai, Taizo
;
Liao, James C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA
Univ Calif Los Angeles, UCLA DOE Inst Genom & Proteom, Los Angeles, CA 90095 USAUniv Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA