SEGMENTATION OF CELLULAR STRUCTURES IN ACTIN TAGGED FLUORESCENCE CONFOCAL MICROSCOPY IMAGES

被引：0

作者：

Matuszewski, B. J. ^{[1
]}

Murphy, M. F. ^{[2
]}

Burton, D. R. ^{[2
]}

Marchant, T. E. ^{[3
]}

Moore, C. J. ^{[3
]}

Histace, A. ^{[4
]}

Precioso, F. ^{[4
,5
]}

机构：

[1] UCLan, Appl Digital Signal & Image Proc Res Ctr, Preston, Lancs, England

[2] Liverpool John Moores Univ, Gen Engn Res Inst, Liverpool, Merseyside, England

[3] Christie NHS Fnd Trust, Northwestern Med Phys, Manchester, Lancs, England

[4] ETIS, CNRS, UMR 8051, Pontoise, France

[5] LIP6, CNRC, UMR 7606, Paris, France

来源：

2011 18TH IEEE INTERNATIONAL CONFERENCE ON IMAGE PROCESSING (ICIP) | 2011年

关键词：

cell segmentation; active contour; geodesic distance; confocal microscopy; topological constraints; GEOMETRIC MODEL; CELLS;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

The paper reports on a novel method for reconstruction of cellular features including cell nuclei and cellular boundaries from actin tagged fluorescence confocal microscopy images. Such reconstruction can provide spatial context for subsequent quantitative analysis of changes to actin organisation and cell morphology in both controlled and stressed cell cultures. The proposed method is fully automatic and is formulated within active contour multiphase level set framework. The derived level set evolution PDEs combine previously proposed curvature and advection flows with propagation flow defined by specially designed set of geodesic distance maps. Additionally the proposed PDEs include additional components to impose known inclusion/exclusion topological constraints between cellular structures. The paper gives an overview of the proposed methodology as well as reports on initial results obtained for monolayer of human prostate cells (PNT2) culture visualised using acting tagged fluorescence confocal microscopy.

引用

页数：4

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