Important parameters for evaluation of antibody avidity by immunosorbent assay

被引：48
作者：
Dimitrov, Jordan D. [1 ]
Lacroix-Desmazes, Sebastien
Kaveri, Srinivas V.
机构：
[1] Ctr Rech Cordeliers, F-75006 Paris, France
来源：
ANALYTICAL BIOCHEMISTRY | 2011年 / 418卷 / 01期
关键词：
Antibody avidity; Immunosorbent assays; Chaotropes; THIOCYANATE ELUTION; VIRUS; IGG; AFFINITY; ANTIGEN;
D O I：
10.1016/j.ab.2011.07.007
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The evaluation of antibody avidity by elution with chaotropic agents is a frequently used approach in research and diagnostics. It provides important information on the functional relevance of antibodies. However, in the literature, there is a large heterogeneity in the experimental settings for the determination of this important parameter. Here, we demonstrate that antibody concentration and the choice of chaotropic agent are critical for the reliable estimation of antibody avidity. (C) 2011 Elsevier Inc. All rights reserved.
引用
收藏
页码：149 / 151
页数：3
相关论文
共 15 条
[1]   Severe pandemic 2009 H1N1 influenza disease due to pathogenic immune complexes [J].
Clara Monsalvo, Ana ;
Batalle, Juan P. ;
Florencia Lopez, M. ;
Krause, Jens C. ;
Klemenc, Jennifer ;
Zea Hernandez, Johanna ;
Maskin, Bernardo ;
Bugna, Jimena ;
Rubinstein, Carlos ;
Aguilar, Leandro ;
Dalurzo, Liliana ;
Libster, Romina ;
Savy, Vilma ;
Baumeister, Elsa ;
Aguilar, Liliana ;
Cabral, Graciela ;
Font, Julia ;
Solari, Liliana ;
Weller, Kevin P. ;
Johnson, Joyce ;
Echavarria, Marcela ;
Edwards, Kathryn M. ;
Chappell, James D. ;
Crowe, James E., Jr. ;
Williams, John V. ;
Melendi, Guillermina A. ;
Polack, Fernando P. .
NATURE MEDICINE, 2011, 17 (02) :195-U225
[2]  
Gassmann C, 1997, J MED VIROL, V51, P242, DOI 10.1002/(SICI)1096-9071(199703)51:3<242::AID-JMV17>3.0.CO
[3]  
2-M
[4]   ARTIFACTS WITH THE THIOCYANATE ELUTION METHOD FOR ESTIMATING RELATIVE ANTIBODY AVIDITY [J].
GRAY, BM ;
SHAW, DR .
JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 157 (1-2) :269-271
[5]   RAPID DIAGNOSIS OF HANTAVIRUS DISEASE WITH AN IGG-AVIDITY ASSAY [J].
HEDMAN, K ;
VAHERI, A ;
BRUMMERKORVENKONTIO, M .
LANCET, 1991, 338 (8779) :1353-1356
[6]   MEASUREMENT OF AVIDITY OF SPECIFIC IGG FOR VERIFICATION OF RECENT PRIMARY RUBELLA [J].
HEDMAN, K ;
ROUSSEAU, SA .
JOURNAL OF MEDICAL VIROLOGY, 1989, 27 (04) :288-292
[7]   ESTIMATING THE RELATIVE AVIDITY OF MUCOSAL IGA FOR ANTIGEN [J].
JONES, CL ;
MACDONALD, RA ;
HOSKING, CS ;
ROBERTON, DM .
JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 105 (01) :111-117
[8]   ANTITETANUS TOXOID ANTIBODIES IN INTRAVENOUS GAMMA-GLOBULIN - AN ALTERNATIVE TO TETANUS IMMUNE GLOBULIN [J].
LEE, DC ;
LEDERMAN, HM .
JOURNAL OF INFECTIOUS DISEASES, 1992, 166 (03) :642-645
[9]   Differential segmental flexibility and reach dictate the antigen binding mode of chimeric IgD and IgM:: Implications for the function of the B cell receptor [J].
Loset, GÅ ;
Roux, KH ;
Zhu, P ;
Michaelsen, TE ;
Sandlie, I .
JOURNAL OF IMMUNOLOGY, 2004, 172 (05) :2925-2934
[10]   THE MEASUREMENT OF RELATIVE ANTIBODY-AFFINITY BY ELISA USING THIOCYANATE ELUTION [J].
MACDONALD, RA ;
HOSKING, CS ;
JONES, CL .
JOURNAL OF IMMUNOLOGICAL METHODS, 1988, 106 (02) :191-194
12