Phospholipidomic identification of potential plasma biomarkers associated with type 2 diabetes mellitus and diabetic nephropathy

被引:100
作者
Zhu, Chao [1 ,2 ]
Liang, Qiong-lin [1 ]
Hu, Ping [3 ]
Wang, Yi-ming [1 ]
Luo, Guo-an [1 ,2 ]
机构
[1] Tsinghua Univ, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Dept Chem, Beijing 100084, Peoples R China
[2] E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China
[3] E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China
关键词
Phospholipids; Phospholipidomic; Type 2 diabetes mellitus; Diabetic nephropathy; Potential biomarkers; PERFORMANCE LIQUID-CHROMATOGRAPHY; MASS-SPECTROMETRY; HYPERGLYCEMIA; HYPERLIPIDEMIA; METABOLISM;
D O I
10.1016/j.talanta.2011.05.036
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Type 2 diabetes mellitus (T2DM) and its attendant complications, such as diabetic nephropathy (DN), impose a significant societal and economic burden. The investigation of discovering potential biomarkers for T2DM and DN will facilitate the prediction and prevention of diabetes. Phospholipids (PLs) and their metabolisms are closely allied to nosogenesis and aggravation of T2DM and DN. The aim of this study is to characterize the human plasma phospholipids in T2DM and ON to identify potential biomarkers of T2DM and DN. Normal phase liquid chromatography coupled with time of flight mass spectrometry (NPLC-TOF/MS) was applied to the plasma phospholipids metabolic profiling of T2DM and DN. The plasma samples from control (n = 30), T2DM subjects (n = 30), and DN subjects (n = 52) were collected and analyzed. The significant difference in metabolic profiling was observed between healthy control group and DM group as well as between control group and ON group by the help of partial least squares discriminant analysis (PLS-DA). PLS-DA and one-way analysis of variance (ANOVA) were successfully used to screen out potential biomarkers from complex mass spectrometry data. The identification of molecular components of potential biomarkers was performed on Ion trap-MS/MS. An external standard method was applied to quantitative analysis of potential biomarkers. As a result, 18 compounds in 7 PL classes with significant regulation in patients compared with healthy controls were regarded as potential biomarkers for T2DM or DN. Among them, 3 DM-specific biomarkers, 8 ON-specific biomarkers and 7 common biomarkers to DM and DN were identified. Ultimately, 2 novel biomarkers, i.e.. PI C18:0/22:6 and SM dC18:0/20:2, can be used to discriminate healthy individuals. T2DM cases and ON cases from each other group. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:1711 / 1720
页数:10
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