Bond-Selective Imaging of Cells by Mid-Infrared Photothermal Microscopy in High Wavenumber Region

被引：51

作者：

Bai, Yeran ^{[1
,2
,3
,4
]}

Zhang, Delong ^{[4
]}

Li, Chen ^{[5
]}

Liu, Cheng ^{[1
,2
]}

Cheng, Ji-Xin ^{[4
]}

机构：

[1] Natl Lab High Power Laser & Phys, Shanghai 201800, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Opt & Fine Mech, Key Lab High Power Laser & Phys, Shanghai 201800, Peoples R China

[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[4] Boston Univ, Photon Ctr, Dept Biomed Engn Elect & Comp Engn, Boston, MA 02215 USA

[5] Purdue Univ, Dept Chem, W Lafayette, IN 47907 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2017年 / 121卷 / 44期

关键词：

DIFFERENCE-FREQUENCY-GENERATION; STIMULATED RAMAN-SCATTERING; MU-M; INFRARED-SPECTROSCOPY; ABSORPTION; PULSES;

D O I：

10.1021/acs.jpcb.7b09570

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Using a visible beam to probe the thermal effect induced by infrared absorption, mid-infrared photothermal (MIP) microscopy allows bond-selective chemical imaging at submicron spatial resolution. Current MIP microscopes cannot reach the high wavenumber region due to the limited tunability of the existing quantum cascade laser source. We extend the spectral range of MIP microscopy by difference frequency generation (DFG) from two chirped femtosecond pulses. Flexible wavelength tuning in both C-D and C-H regions was achieved with mid-infrared power up to 22.1 mW and spectral width of 29.3 cm(-1). Distribution of fatty acid in live human lung cancer cells was revealed by MIP imaging of the C-D bond at 2192 cm(-1).

引用

页码：10249 / 10255

页数：7

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