Optimized Protocols for Generation of Cord Blood-derived Cytokine-induced Killer/Natural Killer Cells

被引：0
作者：
Li, Yang [1 ]
Schmidt-Wolf, Ingo G. H. [4 ]
Wu, Yan-Feng [2 ]
Huang, Shao-Liang [1 ]
Wei, Jing [3 ]
Fang, Jianpei [1 ]
Huang, Ke [1 ]
Zhou, Dun-Hua [1 ]
机构：
[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Pediat, Guangzhou 510120, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Biotherapy Ctr, Guangzhou 510120, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Med Res Ctr, Guangzhou 510120, Guangdong, Peoples R China
[4] Univ Bonn, Dept Internal Med 3, D-53105 Bonn, Germany
来源：
ANTICANCER RESEARCH | 2010年 / 30卷 / 09期
基金：
中国国家自然科学基金;
关键词：
Cytokine-induced killer cell; interleukin; immunotherapy; SEVERE COMBINED IMMUNODEFICIENCY; IMMUNOLOGICAL EFFECTOR-CELLS; CHRONIC MYELOID-LEUKEMIA; VERSUS-HOST-DISEASE; IN-VITRO; T-CELLS; LIGAND; EXPRESSION; EXPANSION; RECEPTOR;
D O I：
暂无
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The efficacy of various combinations of stem cell factor (SCF), FLT3 ligand, interleukin (IL)-2, IL-7 and IL-15 to induce and expand cord blood-derived cytokine-induced killer (CIK) cells was investigated. There were three treatment groups: group A: SCF combined with IL-2, IL-7 and IL-15; group B: SCF, FLT3 ligand combined with IL-2, IL-7 and IL-15, and group C: IL-2, IL-7 and IL-15, the control group. Proliferation rates of CD3(+)CD56(+) CIK cells and CD3(-)CD56(+) natural killer (NK) cells were highest in group B; expansion of CIK cells increased 796.1 +/- 278.5-fold, and that of NK cells increased 36.6 +/- 3.5-fold. All expanded cord blood-derived CIK/NK cells showed cytotoxic activity against the K562 cell line. Interestingly, the cytotoxicity of group A was highest and significantly higher than that of other groups. These protocols might provide an alternative choice for CIK/NK cell expansion.
引用
收藏
页码：3493 / 3499
页数：7
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