Sandwich immunoassay for the prostate specific antigen using a micro-fluxgate and magnetic bead labels

被引：20
作者：
Sun, Xue-cheng [1 ]
Lei, Chong [1 ]
Guo, Lei [1 ]
Zhou, Yong [1 ]
机构：
[1] Shanghai Jiao Tong Univ, Sch Elect Informat & Elect Engn, Dept Micro Nano Elect, Key Lab Thin Film & Microfabricat,Minist Educ, Dongchuan Rd 800, Shanghai 200240, Peoples R China
来源：
MICROCHIMICA ACTA | 2016年 / 183卷 / 08期
基金：
上海市自然科学基金; 中国国家自然科学基金;
关键词：
PSA; Dynabeads; Magnetic field sensor; DC magnetic field; Inductive fringing; FeNi permalloy; Self-assembled monolayer; ELECTROCHEMICAL DETECTION; BIOSENSORS; CANCER; PROTEIN; SYSTEM; SERUM; OXIDE;
D O I：
10.1007/s00604-016-1889-1
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
We describe a micro fluxgate based device with rectangular magnetic core for the determination of prostate specific antigen (PSA) labeled with Dynabeads. A sandwich immunoassay was employed where PSA is captured on a gold film modified with a self-assembled monolayer of antibody. The secondary antibody is labeled with Dynabeads. By applying a DC magnetic fields in the range of 460 to 700 mu T, PSA can be detected with detection limit as low as 0.1 ng mL(-1). This micro fluxgate-based assay offers the advantages of miniaturization, simple and conveniently manipulation, re-usability and stability. In our perception, it offers a viable approach towards clinical determination of PSA or other biomarkers.
引用
收藏
页码：2385 / 2393
页数：9
相关论文
共 34 条
  • [1] An Inexpensive, Fast and Sensitive Quantitative Lateral Flow Magneto-Immunoassay for Total Prostate Specific Antigen
    Barnett, Jacqueline M.
    Wraith, Patrick
    Kiely, Janice
    Persad, Raj
    Hurley, Katrina
    Hawkins, Peter
    Luxton, Richard
    [J]. BIOSENSORS-BASEL, 2014, 4 (03): : 204 - 220
  • [2] A biosensor based on magnetoresistance technology
    Baselt, DR
    Lee, GU
    Natesan, M
    Metzger, SW
    Sheehan, PE
    Colton, RJ
    [J]. BIOSENSORS & BIOELECTRONICS, 1998, 13 (7-8) : 731 - 739
  • [3] Detection of a single magnetic microbead using a miniaturized silicon Hall sensor
    Besse, PA
    Boero, G
    Demierre, M
    Pott, V
    Popovic, R
    [J]. APPLIED PHYSICS LETTERS, 2002, 80 (22) : 4199 - 4201
  • [4] Bhunia AK, 2008, ADV FOOD NUTR RES, V54, P1, DOI 10.1016/S1043-4526(07)00001-0
  • [5] Role of magnetic Fe3O4 graphene oxide in chemiluminescent aptasensors capable of sensing tumor markers in human serum
    Choi, Hyung Kyu
    Lee, Ji Hoon
    [J]. ANALYTICAL METHODS, 2013, 5 (24) : 6964 - 6968
  • [6] Ultrasensitive electrochemical detection of prostate-specific antigen (PSA) using gold-coated magnetic nanoparticles as 'dispersible electrodes'
    Chuah, Kyloon
    Lai, Leo M. H.
    Goon, Ian Y.
    Parker, Stephen G.
    Amal, Rose
    Gooding, J. Justin
    [J]. CHEMICAL COMMUNICATIONS, 2012, 48 (29) : 3503 - 3505
  • [7] Devkota J, 2015, P SOC PHOTO-OPT INS, V9518
  • [8] Microcontact imprinting based surface plasmon resonance (SPR) biosensor for real-time and ultrasensitive detection of prostate specific antigen (PSA) from clinical samples
    Erturk, Gizem
    Ozen, Haluk
    Tumer, M. Askin
    Mattiasson, Bo
    Denizli, Adil
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2016, 224 : 823 - 832
  • [9] Disposable noncompetitive immunosensor for free and total prostate-specific antigen based on capacitance measurement
    Fernández-Sánchez, C
    McNeil, CJ
    Rawson, K
    Nilsson, O
    [J]. ANALYTICAL CHEMISTRY, 2004, 76 (19) : 5649 - 5656
  • [10] Biodetection using magnetically labeled biomolecules and arrays of spin valve sensors (invited)
    Ferreira, HA
    Graham, DL
    Freitas, PP
    Cabral, JMS
    [J]. JOURNAL OF APPLIED PHYSICS, 2003, 93 (10) : 7281 - 7286
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