Preparative separation of grape skin polyphenols by high-speed counter-current chromatography

被引：44

作者：

Luo, Lanxin ^{[1
]}

Cui, Yan ^{[2
]}

Zhang, Shuting ^{[3
,4
]}

Li, Lingxi ^{[2
]}

Li, Yuanyuan ^{[3
]}

Zhou, Peiyu ^{[3
]}

Sun, Baoshan ^{[2
,5
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang 110016, Peoples R China

[2] Shenyang Pharmaceut Univ, Sch Funct Food & Wine, Shenyang 110016, Peoples R China

[3] Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China

[4] Jiamusi Univ, Dept Pharm, Jiamusi 154007, Peoples R China

[5] IP, Inst Natl Invest Agr & Vet, Polo Dois Portos, P-2565191 Dois Portos, Portugal

来源：

FOOD CHEMISTRY | 2016年 / 212卷

关键词：

High-speed counter-current; chromatography; HPLC separation; Polyphenols; Grape skins; PHENOLIC COMPOSITION; ANTIOXIDANT CAPACITY; CYCLIC VOLTAMMETRY; VITIS-VINIFERA; BREAST-CANCER; RED WINE; PROANTHOCYANIDIN; PROCYANIDINS; PURIFICATION; CATECHINS;

D O I：

10.1016/j.foodchem.2016.06.009

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

To develop an efficient method for large preparation of various individual polyphenols from white grape skins (Fernao Pires; Vitis vinifera) by preparative high-speed counter-current chromatography (HSCCC) and preparative-HPLC, an optimized preparative HSCCC condition with two-phase solvent system composed of Hex-EtOAc-H2O (1:50:50, v/v) was used to separate grape skin polyphenols into various fractions. Both the tail-head and head-tail elution modes were used with a flow rate of 3.0 ml/min and a rotary speed of 950 rpm. Afterwards, a preparative-HPLC separation was applied to isolate individual polyphenols in each of the fractions from HSCCC. Total of 7 fractions (Fraction A to G) were obtained from grape skin extract by HSCCC. After preparative-HPLC isolation, fifteen individual compounds were obtained, most of which presented high yields and purity (all over 90%). The HSCCC method followed with preparative-HPLC appeared to be convenient and economical, constituting an efficient strategy for the isolation of grape skin polyphenols. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：712 / 721

页数：10

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