Voltammetric immunoassay for the human blood clotting factor IX by using nanogapped dielectrode junctions modified with gold nanoparticle-conjugated antibody

被引：11
|
作者：
Gopinath, Subash C. B. [1 ,2 ]
Perumal, Veeradasan [3 ]
Rao, Balakrishnan S. [4 ]
Arshad, M. K. Md [1 ]
Voon, Chun Hong [1 ]
Lakshmipriya, Thangavel [1 ]
Haarindraprasad, R. [5 ]
Vijayakumar, Thivina [1 ]
Chen, Yeng [6 ,7 ]
Hashim, Uda [1 ]
机构：
[1] Univ Malaysia Perlis, Inst Nano Elect Engn, Kangar 01000, Malaysia
[2] Univ Malaysia Perlis, Sch Bioproc Engn, Arau 02600, Perlis, Malaysia
[3] Univ Teknol PETRONAS, Ctr Innovat Nanostruct & Nanodevices, Bandar Seri Iskandar 32610, Perak Darul Rid, Malaysia
[4] Univ Sains Islam Malaysia, Fak Kejuruteraan Dan Alam Bina, Nilai 71800, Negeri Sembilan, Malaysia
[5] AIMST Univ, Fac Sci Appl, Bedong 08100, Malaysia
[6] Dept Oral Biol & Craniofacial Sci, Kuala Lumpur 50603, Malaysia
[7] Univ Malaya, Oral Canc Res & Coordinating Ctr, Fac Dent, Kuala Lumpur 50603, Malaysia
来源：
MICROCHIMICA ACTA | 2017年 / 184卷 / 10期
关键词：
Electrochemical assay; Biosensor; Coagulation; Impedance; Serumproteins; BIOMOLECULAR INTERACTIONS; APTAMERS; COAGULATION; SENSITIVITY; BIOSENSOR; BLOCKING;
D O I：
10.1007/s00604-017-2389-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The authors describe an electrochemical method for the determination of the blood clotting factor IX (FIX). A nanogapped dielectrode (with a < 100 nm junction) was modified with antibody against FIX, and the resulting system was characterized by both impedance spectroscopy and voltammetry. In order to attain the improved sensitivity, gold nanoparticles were electrostatically attached to FIX. The current to voltage (I-V) measurement was carried out from 1 V to 5 V, where the entire calibration plot with 5 V was taken. This results in a limit of detection as low of 1 pM, which is much lower compared to the real concentration of FIX(87 nM) in human blood serum. The analytical range extends from 1 pM to 0.1 mu M. The electrode is highly specific over other serum proteins.
引用
收藏
页码：3739 / 3745
页数：7
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