A novel genetic marker to differentiate feline herpesvirus type 1 field isolates

被引:6
作者
Hamano, M
Maeda, K
Kai, K
Mochizuki, M
Tohya, Y
Akashi, H
机构
[1] Yamaguchi Univ, Fac Agr, Dept Vet Microbiol, Yamaguchi 7538515, Japan
[2] Kyoritsu Seiyaku Corp, Lab Clin Microbiol, Chiyoda Ku, Tokyo 1020073, Japan
[3] Univ Tokyo, Dept Vet Microbiol, Grad Sch Agr & Life Sci, Bunkyo Ku, Tokyo 1138657, Japan
关键词
epidemiology; feline herpesvirus type 1; genetic marker;
D O I
10.1016/j.vetmic.2004.12.028
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Five recent field isolates of feline herpesvirus type I (FHV-1) were compared by digestion with a restriction endonuclease, SalI or MluI. The SalI digestion showed a potentially useful difference in one isolate 00-035 that had an approximately 3.0 kbp fragment instead of a 2.6 kbp fragment in the other strains. After cloning the 3.0 and 2.6 kbp fragments, the nucleotide sequences were analyzed. The result showed that the 3.0 kbp fragment of 00-035 included a complete open reading frame of the herpes simplex virus 1 (HSV-1) homologue of the UL17 gene and a 5'-part of UL16 gene and that only one nucleotide substitution was found in the 5'-region of UL17 gene where the SalI site of the 2.6 kbp fragment locates. Based on these nucleotide sequences, two PCR primers were designed to amplify the region around the SalI site in the UL17 gene and the PCR was carried out using 78 field isolates from various parts of Japan. The SalI digestion of the PCR products revealed an interesting profile in that the genotype without the SalI site in UL17 gene was dominant in Tottori and Yamagata prefectures (69% and 75%, respectively) but minor in the other regions of Japan (0-10%). These results suggest that the SalI digestion method described in the present study can be used as a genetic marker to differentiate some FHV-1 field isolates and this is the first report that showed different distributions of FHV-1 genotypes using the novel genetic marker. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:195 / 200
页数:6
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