An Aptamer-based Fluorescence Biosensor for Insulin Detection Based on Graphene Oxide

被引：1
|
作者：
Jiang Li-Ying [1 ]
Xiao Xiao-Nan [1 ]
Zhou Peng-Lei [1 ]
Zhang Pei [1 ]
Yan Yan-Xia [1 ]
Jiang Su-Xia [1 ]
Chen Qing-Hua [1 ]
机构：
[1] Zhengzhou Univ Light Ind, Inst Elect & Informat Engn, Zhengzhou 450002, Peoples R China
来源：
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2016年 / 44卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Fluorophore; Aptamer; Graphene oxide; Fluorescence aptamer biosensor; Insulin; BLOOD-SERUM; ELECTRODE;
D O I：
10.11895/j.issn.0253-3820.150534
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
By using fluorophore (FAM) labeled aptamers as recognition elements and graphene oxide as a quencher, a highly selective and sensitive sensor was constructed for the fast and accurate detection of insulin. After the aptamer binding with graphene oxide, fluorescence will be quenched and fluorescence intensity disappeared; after addition of insulin, the solution fluorescence was restored. Based on the fact, we established a method for the determination of insulin concentration by measuring the fluorescence intensity. The results showed that the concentration of insulin in the range of 5 x 10(-8) - 1 x 10(-5) mol/L had a good linear relationship with the fluorescence intensity. The detection limit was 10 nmol/L. This method of detecting insulin had obvious advantages of fast detection speed, high selectivity and low detection limit.
引用
收藏
页码：310 / 314
页数：5
相关论文
共 24 条
  • [1] Electrocatalytic determination of traces of insulin using a novel silica nanoparticles-Nafion modified glassy carbon electrode
    Amini, Nader
    Gholivand, Mohammad Bagher
    Shamsipur, Mojtaba
    [J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2014, 714 : 70 - 75
  • [2] DONG Hao, 2014, CHINESE J LUMINESCEN, V35, P767
  • [3] A supported liquid membrane for microextraction of insulin, and its determination with a pencil graphite electrode modified with RuO2-graphene oxide
    Ensafi, Ali Asghar
    Khoddami, Elaheh
    Rezaei, Behzad
    Jafari-Asl, Mehdi
    [J]. MICROCHIMICA ACTA, 2015, 182 (9-10) : 1599 - 1607
  • [4] Development of Graphene-based Optical Biosensor
    Gao Yuan
    Li Yan
    Su Xing-Guang
    [J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2013, 41 (02) : 174 - 180
  • [5] Development of an electrochemical insulin sensor based on the insulin-linked polymorphic region
    Gerasimov, Jennifer Y.
    Schaefer, Cody S.
    Yang, Weiwei
    Grout, Rebecca L.
    Lai, Rebecca Y.
    [J]. BIOSENSORS & BIOELECTRONICS, 2013, 42 : 62 - 68
  • [6] [黄河洲 Huang Hezhou], 2014, [发光学报, Chinese Journal of Luminescence], V35, P142
  • [7] Silica gel modified carbon paste electrode for electrochemical detection of insulin
    Jaafariasl, Mehdi
    Shams, Esmaeil
    Amini, Mohammad Kazem
    [J]. ELECTROCHIMICA ACTA, 2011, 56 (11) : 4390 - 4395
  • [8] Ultrasensitive Label Free Electrical Detection of Insulin in Neat Blood Serum
    Luo, Xiliang
    Xu, Mengyun
    Freeman, Charlotte
    James, Tim
    Davis, Jason J.
    [J]. ANALYTICAL CHEMISTRY, 2013, 85 (08) : 4129 - 4134
  • [9] Insulin-binding aptamer-conjugated graphene oxide for insulin detection
    Pu, Ying
    Zhu, Zhi
    Han, Da
    Liu, Huixia
    Liu, Jun
    Liao, Jie
    Zhang, Kejing
    Tan, Weihong
    [J]. ANALYST, 2011, 136 (20) : 4138 - 4140
  • [10] Electrocatalytic oxidation and determination of insulin at nickel oxide nanoparticles-multiwalled carbon nanotube modified screen printed electrode
    Rafiee, Banafsheh
    Fakhari, Ali Reza
    [J]. BIOSENSORS & BIOELECTRONICS, 2013, 46 : 130 - 135
123