Effects of polyamines on apoptosis induced by simulated ischemia/reperfusion injury in cultured neonatal rat cardiomyocytes

被引：37

作者：

Han, Liping ^{[1
]}

Xu, Changqing ^{[1
]}

Jiang, Chunming ^{[1
]}

Li, Hongzhu ^{[1
]}

Zhang, Weihua ^{[1
]}

Zhao, Yajun ^{[1
]}

Zhang, Li ^{[1
]}

Zhang, Yanqiao ^{[1
]}

Zhao, Weiming ^{[1
]}

Yang, Baofeng ^{[1
]}

机构：

[1] Harbin Med Univ, Dept Pathophysiol, Harbin 150086, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2007年 / 31卷 / 11期

基金：

中国国家自然科学基金;

关键词：

polyamine; heart; ischemia; reperfusion; apoptosis;

D O I：

10.1016/j.cellbi.2007.05.015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We incubated neonatal Sprague-Dawley rat cardiornyocytes in primary culture in a medium simulating ischermia (consisting of hypoxia plus serum deprivation) for 2 It, then re-incubated them for 24 It in normal culture medium to establish a model of simulated ischemia/reperfusion (I/R) injury. Apoptotic cell death was measured by MTT assay, TUNEL staining and flow cytometry. Morphological alterations were assessed by transmission electron microscopy, the expression of caspases-3 and -9 and Bcl-2 and the release of cytochrome c by Western blotting, and the intracellular free-calcium concentration ([Ca2+](i)) by laser confocal scanning microscopy. The results showed that pretreatment with 10 mu mol/l spermine or spermidine significantly inhibited apoptosis in the I/R cells, suppressed the expression of caspases-3 and -9 and cytochrome c release, up-regulated Bcl-2 expression and decreased [Ca2+](i). However, pretreatment with 10 mu mol/l putrescine had the opposite effects. Evidence for this "double-edged" effect of polyamines on apoptosis in I/R-injured cardiomyocytes is presented for the first time. It may suggest a novel pharmacological target for preventing and treating cardiac ischemia/reperfusion injury. (c) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：1345 / 1352

页数：8

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