Immune responses to a single dose of the AZD1222/Covishield vaccine in health care workers

被引：36

作者：

Jeewandara, Chandima ^{[1
]}

Kamaladasa, Achala ^{[1
]}

Pushpakumara, Pradeep Darshana ^{[1
]}

Jayathilaka, Deshni ^{[1
]}

Aberathna, Inoka Sepali ^{[1
]}

Danasekara, Danasekara Rallage Saubhagya Rasikangani ^{[1
]}

Guruge, Dinuka ^{[2
]}

Ranasinghe, Thushali ^{[1
]}

Dayarathna, Shashika ^{[1
]}

Pathmanathan, Thilagaraj ^{[1
]}

Somathilake, Gayasha ^{[1
]}

Madhusanka, Panambara Arachchige Deshan ^{[1
]}

Ramu, Shyrar Tanussiya ^{[1
]}

Jayadas, Tibutius Thanesh Pramanayagam ^{[1
]}

Kuruppu, Heshan ^{[1
]}

Wijesinghe, Ayesha ^{[1
]}

Nimasha, Herath Mudiyanselage Thashmi ^{[1
]}

Milroy, Dushantha ^{[3
]}

Nandasena, Achini Anuja ^{[4
]}

Sanjeewani, Poththawela Kankanam Gamage Nilanka ^{[4
]}

Wijayamuni, Ruwan ^{[2
]}

Samaraweera, Sudath ^{[5
]}

Schimanski, Lisa ^{[6
,7
]}

Tan, T. K. ^{[6
,7
]}

Dong, Tao ^{[6
,7
]}

Ogg, Graham S. ^{[6
]}

Townsend, Alain ^{[6
,7
]}

Malavige, Gathsaurie Neelika ^{[1
,6
]}

机构：

[1] Univ Sri Jayewardenepura, Dept Immunol & Mol Med, Allergy Immunol & Cell Biol Unit, Nugegoda, Sri Lanka

[2] Colombo Municipal Council, Colombo, Sri Lanka

[3] Base Hosp, Dambadeniya, Sri Lanka

[4] Univ Sri Jayewardenepura, Dept Family Med, Sri Jayewardenepura Kott, Sri Lanka

[5] Minist Hlth Sri Lanka, Epidemiol Unit, Colombo, Sri Lanka

[6] Univ Oxford, MRC Weatherall Inst Mol Med, MRC Human Immunol Unit, Oxford, England

[7] Univ Oxford, Chinese Acad Med Sci Oxford Inst, Ctr Translat Immunol, Oxford, England

来源：

NATURE COMMUNICATIONS | 2021年 / 12卷 / 01期

基金：

英国医学研究理事会;

关键词：

DISEASE SEVERITY;

D O I：

10.1038/s41467-021-24579-7

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Several COVID-19 vaccines have received emergency approval. Here we assess the immunogenicity of a single dose of the AZD1222 vaccine, at one month, in a cohort of health care workers (HCWs) (629 naive and 26 previously infected). 93.4% of naive HCWs seroconverted, irrespective of age and gender. Haemagglutination test for antibodies to the receptor binding domain (RBD), surrogate neutralization assay (sVNT) and ex vivo IFN gamma ELISpot assays were carried out in a sub-cohort. ACE2 blocking antibodies (measured by sVNT) were detected in 67/69 (97.1%) of naive HCWs. Antibody levels to the RBD of the wild-type virus were higher than to RBD of B.1.1.7, and titres to B.1.351 were very low. Ex vivo T cell responses were observed in 30.8% to 61.7% in naive HCWs. Previously infected HCWs, developed significantly higher (p<0.0001) ACE2 blocking antibodies and antibodies to the RBD for the variants B.1.1.7 and B.1.351. This study shows high seroconversion after one vaccine dose, but also suggests that one vaccine dose may be insufficient to protect against emerging variants. Here the authors show in a large cohort of healthcare workers that one dose of the AZD1222 vaccine seroconverts 92.9% of vaccinees, irrespective of age and gender, and results in high antibody titers to wild-type SARS-CoV-2 but only low titers to the B.1.351 variant.

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页数：9

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