Transplantation of mesenchymal stem cells overexpressing IL10 attenuates cardiac impairments in rats with myocardial infarction

被引：33

作者：

Meng, Xin ^{[1
]}

Li, Jianping ^{[2
]}

Yu, Ming ^{[1
]}

Yang, Jian ^{[3
]}

Zheng, Minjuan ^{[1
]}

Zhang, Jinzhou ^{[3
]}

Sun, Chao ^{[1
]}

Liang, Hongliang ^{[3
]}

Liu, Liwen ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Ultrasonog, 127 Changle West Rd, Xian 710032, Shaanxi, Peoples R China

[2] Fourth Mil Med Univ, Dept Radiat Oncol, Xijing Hosp, Xian, Shaanxi, Peoples R China

[3] Fourth Mil Med Univ, Dept Cardiovasc Surg, Xijing Hosp, 127 Changle West Rd, Xian 710032, Shaanxi, Peoples R China

来源：

JOURNAL OF CELLULAR PHYSIOLOGY | 2018年 / 233卷 / 01期

基金：

中国国家自然科学基金;

关键词：

cardiac injury; inflammation; interleukin-10; mesenchymal stem cell; myocardial infarction; DOUBLE-BLIND; MODEL; INTERLEUKIN-10; DISEASE; HEART; IL-10; AKT; PROLIFERATION; PRETREATMENT; INFLAMMATION;

D O I：

10.1002/jcp.25919

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Mesenchymal stem cell (MSC) has been well known to exert therapeutic potential for patients with myocardial infarction (MI). In addition, interleukin-10 (IL10) could attenuate MI through suppressing inflammation. Thus, the combination of MSC implantation with IL10 delivery may extend health benefits to ameliorate cardiac injury after MI. Here we established overexpression of IL10 in bone marrow-derived MSC through adenoviral transduction. Cell viability, apoptosis, and IL10 secretion under ischemic challenge in vitro were examined. In addition, MSC was transplanted into the injured hearts in a rat model of MI. Four weeks after the MI induction, MI, cardiac functions, apoptotic cells, and inflammation cytokines were assessed. In response to in vitro oxygen-glucose deprivation (OGD), IL10 overexpression in MSC (Ad.IL10-MSC) enhanced cell viability, decreased apoptosis, and increased IL10 secretion. Consistently, the implantation of Ad.IL10-MSCs into MI animals resulted in more reductions in myocardial infarct size, cardiac impairment, and cell apoptosis, compared to the individual treatments of either MSC or IL10 administration. Moreover, the attenuation of both systemic and local inflammations was most prominent for Ad.IL10-MSC treatment. IL10 overexpression and MSC may exert a synergistic anti-inflammatory effect to alleviate cardiac injury after MI.

引用

页码：587 / 595

页数：9

共 40 条

[1] Interleukin-1 Blockade With Anakinra to Prevent Adverse Cardiac Remodeling After Acute Myocardial Infarction (Virginia Commonwealth University Anakinra Remodeling Trial [VCU-ART] Pilot Study) [J].