Structure and gene cluster of the O-antigen of Escherichia coli O41

被引：4
|
作者：
Zhu, Hongfei [2 ]
Perepelov, Andrei V. [1 ]
Senchenkova, Sof'ya N. [1 ]
Shashkov, Alexander S. [1 ]
Wang, Lei [2 ]
Knirel, Yuriy A. [1 ]
机构：
[1] Russian Acad Sci, ND Zelinsky Inst Organ Chem, Moscow 119991, Russia
[2] Nankai Univ, TEDA, TEDA Sch Biol Sci & Biotechnol, Tianjin 300071, Peoples R China
来源：
CARBOHYDRATE RESEARCH | 2012年 / 349卷
基金：
俄罗斯基础研究基金会; 美国国家科学基金会;
关键词：
Escherichia coli; Bacterial polysaccharide structure; O-antigen; Lipopolysaccharide; O-antigen gene cluster; BIOSYNTHESIS; POLYSACCHARIDES; IDENTIFICATION; ORGANIZATION;
D O I：
10.1016/j.carres.2011.12.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The acidic O-polysaccharide (O-antigen) of Escherichia coli O41 was studied by sugar analysis along with 1D and 2D H-1 and C-13 NMR spectroscopy, and the following structure of the branched hexasaccharide repeating unit was established: beta-D-GlcpA 1 down arrow 4 -> 3)-alpha-D-Galp-(1 -> 3)-alpha-L-Fucp-(1 -> 4)-beta-D-GlcpNAc-(1 -> 3)-alpha-L-Fucp-(1 -> 3)->beta-D-GlcpNAc-(1 -> This structure is unique among the known structures of bacterial polysaccharides. The O-antigen gene cluster of E. coli O41 was sequenced. The gene functions were tentatively assigned by a comparison with sequences in the available databases and found to be in full agreement with the E. coli O41 O-polysaccharide structure. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码：86 / 89
页数：4
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