Novel durum wheat genes up-regulated in response to a combination of heat and drought stress

被引:64
|
作者
Rampino, Patrizia [1 ]
Mita, Giovanni [2 ]
Fasano, Pasqua [1 ]
Borrelli, Grazia Maria [3 ]
Aprile, Alessio [1 ]
Dalessandro, Giuseppe [1 ]
De Bellis, Luigi [1 ]
Perrotta, Carla [1 ]
机构
[1] Univ Salento, Dipartimento Sci & Tecnol Biol & Ambientali, I-73100 Lecce, Italy
[2] CNR, Ist Prod Alimentari, I-73100 Lecce, Italy
[3] Ctr Ric Cerealicoltura CRA CER, I-71122 Foggia, Italy
关键词
Heat stress; Drought; Combined stress; Durum wheat; cDNA-AFLP; CDNA-AFLP; ABIOTIC STRESS; TOLERANCE; EXPRESSION; ARABIDOPSIS; SALINITY; PLANTS; FIELD; SHOCK; SALT;
D O I
10.1016/j.plaphy.2012.04.006
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We report the effect of heat, drought and combined stress on the expression of a group of genes that are up-regulated under these conditions in durum wheat (Triticum turgidum subsp. durum) plants. Modulation of gene expression was studied by cDNA-AFLP performed on RNAs extracted from flag leaves. By this approach, we identified several novel durum wheat genes whose expression is modulated under different stress conditions. We focused on a group of hitherto undescribed up-regulated genes in durum wheat, among these, 7 are up-regulated by heat, 8 by drought stress, 15 by combined heat and drought stress, 4 are up-regulated by both heat and combined stress, and 3 by both drought and combined stress. The functional characterization of these genes will provide new data that could help the developing of strategies aimed at improving durum wheat tolerance to field stress. (C) 2012 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:72 / 78
页数:7
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