Determination of Idarubicin Using CdTe Quantum Dots as Fluorescence Probes

A simple and fast method for the determination of Idarubicin (IDA) was developed based on the fluorescence quenching effect of IDA for 3-mercaptopropionic acid-capped CdTe quantum dots. Under optimum conditions, the calibration plot of F-0/F versus the concentration of IDA was linear in the range of 1-200 mu M with correlation coefficient of 0.9969. The limit of detection was 0.2 mu M. The recoveries of IDA standard solution spiked with sample were in the range of 99.75-100.9% with the relative standard deviations less than 5.0%. The method was applied to the determination of IDA in its commercial powder-injection sample and the result agreed with the claimed values. The mechanism of the fluorescence quenching was discussed.