The suppressive effect of mesenchymal stromal cells on T cell proliferation is conserved in old age

被引:21
|
作者
Landgraf, Katja [1 ]
Brunauer, Regina [1 ]
Lepperdinger, Guenter [1 ]
Grubeck-Loebenstein, Beatrix [1 ]
机构
[1] Austrian Acad Sci, Inst Biomed Aging Res, A-6020 Innsbruck, Austria
关键词
Aging; Mesenchymal stromal cells; Interleukin-6; Immunosuppression; Graft versus host disease; Human; VERSUS-HOST-DISEASE; STEM-CELLS; INHIBIT; DIFFERENTIATION; RESPONSES; ANERGY; PLAYS;
D O I
10.1016/j.trim.2011.06.007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Mesenchymal stromal cells (MSC) have become a useful tool in curing graft versus host disease (GVHD) after transplantation. No information is presently available whether the immunosuppressive properties of this cell type are maintained in old age. It was therefore the aim of our study to analyze the immunoregulatory effect of MSC on peripheral blood mononuclear cells (PBMC) in old age. We studied the proliferation, activation and cytokine production of PBMC following co-culture with MSC from young (<30 years) and old (>61 years) donors. Our results demonstrate that MSC from elderly donors exhibit the same suppressive effects on T cell proliferation as their young counterparts. In both age groups T cell activation was not influenced by co-culture with MSC from young and elderly donors. With the exception of IL-6, cytokine production by unstimulated or stimulated PBMC was also not affected by MSC from either age group. IL-6 production was increased during co-culture of PBMC and MSC and was higher when MSC from elderly donors were used. After PHA stimulation, however, this age-specific difference was balanced and appeared even. As high IL-6 production is a prerequisite for an effective suppression of T cell proliferation, MSC can be considered a powerful tool for immunoregulatory therapies in old age. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:167 / 172
页数:6
相关论文
共 50 条
  • [41] Essential Components for Ex Vivo Proliferation of Mesenchymal Stromal Cells
    Fekete, Natalie
    Rojewski, Markus Thomas
    Lotfi, Ramin
    Schrezenmeier, Hubert
    TISSUE ENGINEERING PART C-METHODS, 2014, 20 (02) : 129 - 139
  • [42] Unexpected role for lymph node stromal cells in suppressing T cell proliferation
    Luther, S.
    Scarpellino, L.
    Flavell, S.
    Tomei, A.
    Swartz, M.
    Nelson, P.
    Heikenwaelder, M.
    Acha-Orbea, H.
    Buckley, C.
    Siegert, S.
    ALLERGOLOGIE, 2011, 34 (02) : 77 - 78
  • [43] Cell Identity, Proliferation, and Cytogenetic Assessment of Equine Umbilical Cord Blood Mesenchymal Stromal Cells
    Alizadeh, Amir H. i
    Briah, Ritesh
    Villagomez, Daniel A. F.
    King, William A.
    Koch, Thomas G.
    STEM CELLS AND DEVELOPMENT, 2018, 27 (24) : 1729 - 1738
  • [44] Mesenchymal Stromal Cells from Fernale Donors Enhance Breast Cancer Cell Proliferation in vitro
    Pasanen, Ilkka
    Pietila, Mika
    Lehtonen, Siri
    Lehtilahti, Elisa
    Hakkarainen, Tanja
    Sequeiros, Roberto Blanco
    Lehenkari, Petri
    Kuvaja, Paula
    ONCOLOGY, 2015, 88 (04) : 214 - 225
  • [45] Multispectral characterisation of Mesenchymal Stem/Stromal Cells: Age, Cell cycle, Senescence, and Pluripotency
    Campbell, Jared M.
    Habibalahi, Abbas
    Mahbub, Saabah B.
    Paton, Sharon
    Gronthos, Stan
    Goldys, Ewa M.
    LABEL-FREE BIOMEDICAL IMAGING AND SENSING (LBIS) 2020, 2020, 11251
  • [46] The Effect of Cell Culture Passage on the Efficacy of Mesenchymal Stromal Cells as a Cell Therapy Treatment
    Carmona-Luque, M. Dolores
    Ballesteros-Ribelles, Antonio
    Millan-Lopez, Alejandro
    Blanco, Alfonso
    Nogueras, Sonia
    Herrera, Concha
    JOURNAL OF CLINICAL MEDICINE, 2024, 13 (09)
  • [47] Simvastatin Modulates Mesenchymal Stromal Cell Proliferation and Gene Expression
    Zanette, Dalila Luciola
    Cetrulo Lorenzi, Julio Cesar
    Panepucci, Rodrigo Alexandre
    Bonini Palma, Patricia Vianna
    dos Santos, Daiane Fernanda
    Prata, Karen Lima
    Silva, Wilson Arajo, Jr.
    PLOS ONE, 2015, 10 (04):
  • [48] Human mesenchymal stromal cells transiently increase cytokine production by activated T cells before suppressing T-cell proliferation: effect of interferon-γ and tumor necrosis factor-α stimulation
    Cuerquis, Jessica
    Romieu-Mourez, Raphaelle
    Francois, Moira
    Routy, Jean-Pierre
    Young, Yoon Kow
    Zhao, Jing
    Eliopoulos, Nicoletta
    CYTOTHERAPY, 2014, 16 (02) : 191 - 202
  • [49] A Mesenchymal Stromal Cell Gene Signature for Donor Age
    Alves, Hugo
    van Ginkel, Jetty
    Groen, Nathalie
    Hulsman, Marc
    Mentink, Anouk
    Reinders, Marcel
    van Blitterswijk, Clemens
    de Boer, Jan
    PLOS ONE, 2012, 7 (08):
  • [50] Mesenchymal stromal cells in hematopoietic cell transplantation
    Burnham, Andre J.
    Daley-Bauer, Lisa P.
    Horwitz, Edwin M.
    BLOOD ADVANCES, 2020, 4 (22) : 5877 - 5887